Yang Zhai1, Jiu-Xiang Zhu1,2, Tai-Meng Tan1,2, Jian-Ping Xu1, Ai-Rong Shen2,3, Xie-Bin Yang1,2, Ji-Lie Li2, Liang-Bin Zeng4, Lin Wei5. 1. Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, No.348 Xianjiahu West Road, Changsha, 410205, Hunan, China. 2. Hunan Provincial Key Laboratory of Forestry Biotechnology, Central South University of Forestry and Technology, Changsha, 410004, China. 3. Hunan Academy of Forestry, Changsha, 410004, China. 4. Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, No.348 Xianjiahu West Road, Changsha, 410205, Hunan, China. zengliangbin@caas.cn. 5. Institute of Plant Protection, Hunan Academy of Agricultural Sciences, No.726 Yuanda 2nd Road, Changsha, 410125, Hunan, China. nkyweilin@163.com.
Abstract
OBJECTIVE: The aim of this study is to evaluate the efficacy of the strain Paenibacillus polymyxa HX-140, isolated from the rhizosphere soil of rape, to control Fusarium wilt of cucumber seedlings caused by Fusarium oxysporum f. sp. cucumerinum. RESULTS: Strain HX-140 was able to produce protease, cellulase, β-1,3-glucanase and antifungal volatile organic compounds. An in vitro dual culture test showed that strain HX-140 exhibited broad spectrum antifungal activity against soil-borne plant pathogenic fungi. Strain HX-140 also reduced the infection of Fusarium wilt of cucumber seedlings by 55.6% in a greenhouse pot experiment. A field plot experiment confirmed the biocontrol effects and further revealed that antifungal activity was positively correlated with inoculum size by the root-irrigation method. Here, inoculums at 106 107 and 108 cfu/mL of HX-140 bacterial suspension reduced the incidence of Fusarium wilt of cucumber seedling by 19.5, 41.1, and 50.9%, respectively. CONCLUSIONS: Taken together, our results suggest that P. polymyxa HX-140 has significant potential in the control of Fusarium wilt and possibly other fungal diseases of cucumber.
OBJECTIVE: The aim of this study is to evaluate the efficacy of the strain Paenibacillus polymyxaHX-140, isolated from the rhizosphere soil of rape, to control Fusarium wilt of cucumber seedlings caused by Fusarium oxysporum f. sp. cucumerinum. RESULTS: Strain HX-140 was able to produce protease, cellulase, β-1,3-glucanase and antifungal volatile organic compounds. An in vitro dual culture test showed that strain HX-140 exhibited broad spectrum antifungal activity against soil-borne plant pathogenic fungi. Strain HX-140 also reduced the infection of Fusarium wilt of cucumber seedlings by 55.6% in a greenhouse pot experiment. A field plot experiment confirmed the biocontrol effects and further revealed that antifungal activity was positively correlated with inoculum size by the root-irrigation method. Here, inoculums at 106 107 and 108 cfu/mL of HX-140 bacterial suspension reduced the incidence of Fusarium wilt of cucumber seedling by 19.5, 41.1, and 50.9%, respectively. CONCLUSIONS: Taken together, our results suggest that P. polymyxa HX-140 has significant potential in the control of Fusarium wilt and possibly other fungal diseases of cucumber.