Yuebin Lin1, Ya Yang2, Yongjuan Zhao2, Fan Gao1, Xin Guo1, Minhui Yang1, Qingxiang Hong1, Zhongmei Yang1, Juan Dai1, Changjiang Pan3. 1. Faculty of Mechanical and Material Engineering, Huaiyin Institute of Technology, Huai'an, 223003, China. 2. The Affiliated Huai'an Hospital of Xuzhou Medical University, Huai'an, 223003, China. 3. Faculty of Mechanical and Material Engineering, Huaiyin Institute of Technology, Huai'an, 223003, China. panchangjiang@hyit.edu.cn.
Abstract
The in vivo fast degradation and poor biocompatibility are two major challenges of the magnesium alloys in the field of artificial bone materials. In this study, graphene oxide (GO) was first functionalized by chitosan (GOCS) and then immobilized on the magnesium alloy surface, finally the complex of heparin and bone morphogenetic protein 2 was incorporated on the modified surface to synergistically improve the corrosion resistance, anticoagulation, and osteogenesis. Apart from an excellent hydrophilicity after the surface modification, a sustained heparin and BMP2 release over 14 days was achieved. The corrosion resistance of the modified magnesium alloy was significantly better than that of the control according to the results of electrochemical tests. Moreover, the corrosion rate was also significantly reduced in contrast to the control. The modified magnesium alloy not only had excellent anticoagulation, but also can significantly promote osteoblast adhesion and proliferation, upregulate the expression of alkaline phosphatase and osteocalcin, and enhance mineralization. Therefore, the method of the present study can be used to simultaneously improve the corrosion resistance and biocompatibility of the magnesium alloys targeted for the orthopedic applications.
The in vivo fast degradation and poor biocompatibility are two major challenges of the n class="Chemical">magnesiumalloys in the field of artificial bone materials. In this study, graphene oxide (GO) was first functionalized by chitosan (GOCS) and then immobilized on the magnesiumalloy surface, finally the complex of heparin and bone morphogenetic protein 2 was incorporated on the modified surface to synergistically improve the corrosion resistance, anticoagulation, and osteogenesis. Apart from an excellent hydrophilicity after the surface modification, a sustained heparin and BMP2 release over 14 days was achieved. The corrosion resistance of the modified magnesiumalloy was significantly better than that of the control according to the results of electrochemical tests. Moreover, the corrosion rate was also significantly reduced in contrast to the control. The modified magnesiumalloy not only had excellent anticoagulation, but also can significantly promote osteoblast adhesion and proliferation, upregulate the expression of alkaline phosphatase and osteocalcin, and enhance mineralization. Therefore, the method of the present study can be used to simultaneously improve the corrosion resistance and biocompatibility of the magnesiumalloys targeted for the orthopedic applications.
Authors: Su Ryon Shin; Yi-Chen Li; Hae Lin Jang; Parastoo Khoshakhlagh; Mohsen Akbari; Amir Nasajpour; Yu Shrike Zhang; Ali Tamayol; Ali Khademhosseini Journal: Adv Drug Deliv Rev Date: 2016-03-29 Impact factor: 15.470