Nayara Sousa da Silva1, Nathália Kelly Araújo2, Alessandra Daniele-Silva3, Johny Wysllas de Freitas Oliveira4, Júlia Maria de Medeiros5, Renata Mendonça Araújo6, Leandro De Santis Ferreira2, Hugo Alexandre Oliveira Rocha7, Arnóbio Antônio Silva-Junior2, Marcelo Sousa Silva8,9, Matheus de Freitas Fernandes-Pedrosa2. 1. Postgraduate Program in Pharmacy, Faculty of Pharmacy, Federal University of Rio Grande do Norte, Natal 59012-570, Brazil. 2. Department of Pharmacy, Faculty of Pharmacy, Federal University of Rio Grande do Norte, Natal 59012-570, Brazil. 3. Postgraduate Program in Development and Technological Innovation in Medicines, Bioscience Center, Federal University of Rio Grande do Norte, Natal 59072-970, Brazil. 4. Postgraduate Program in Biochemistry, Bioscience Center, Federal University of Rio Grande do Norte, Natal 59072-970, Brazil. 5. Postgraduate Program in Chemical Engineering, Technology Center, Federal University of Rio Grande do Norte, Natal 59072-970, Brazil. 6. Chemistry Institute, Federal University of Rio Grande do Norte, Natal 59072-970, Brazil. 7. Department of Biochemistry, Bioscience Center, Federal University of Rio Grande do Norte, Natal 59072-970, Brazil. 8. Department of Clinical and Toxicological Analysis, Faculty of Pharmacy, Federal University of Rio Grande do Norte, Natal 59012-570, Brazil. 9. Global Health and Tropical Medicine, Institute of Hygiene and Tropical Medicine, University of Nova Lisboa, 1099-085 Lisbon, Portugal.
Abstract
The global rise of infectious disease outbreaks and the progression of microbial resistance reinforce the importance of researching new biomolecules. Obtained from the hydrolysis of chitosan, chitooligosaccharides (COSs) have demonstrated several biological properties, including antimicrobial, and greater advantage over chitosan due to their higher solubility and lower viscosity. Despite the evidence of the biotechnological potential of COSs, their effects on trypanosomatids are still scarce. The objectives of this study were the enzymatic production, characterization, and in vitro evaluation of the cytotoxic, antibacterial, antifungal, and antiparasitic effects of COSs. NMR and mass spectrometry analyses indicated the presence of a mixture with 81% deacetylated COS and acetylated hexamers. COSs demonstrated no evidence of cytotoxicity upon 2 mg/mL. In addition, COSs showed interesting activity against bacteria and yeasts and a time-dependent parasitic inhibition. Scanning electron microscopy images indicated a parasite aggregation ability of COSs. Thus, the broad biological effect of COSs makes them a promising molecule for the biomedical industry.
The global rise of infectious disease outbreaks and the progression of microbial resistance reinforce the importance of researpan class="Chemical">ching new biomolecules. Obtained from the hydrolysis of chitosan, chitooligosaccharides (COSs) have demonstrated several biological properties, including antimicrobial, and greater advantage over chitosan due to their higher solubility and lower viscosity. Despite the evidence of the biotechnological potential of COSs, their effects on trypanosomatids are still scarce. The objectives of this study were the enzymatic production, characterization, and in vitro evaluation of the cytotoxic, antibacterial, antifungal, and antiparasitic effects of COSs. NMR and mass spectrometry analyses indicated the presence of a mixture with 81% deacetylated COS and acetylated hexamers. COSs demonstrated no evidence of cytotoxicity upon 2 mg/mL. In addition, COSs showed interesting activity against bacteria and yeasts and a time-dependent parasitic inhibition. Scanning electron microscopy images indicated a parasite aggregation ability of COSs. Thus, the broad biological effect of COSs makes them a promising molecule for the biomedical industry.
Authors: Imelda Galván Márquez; Jones Akuaku; Isabel Cruz; James Cheetham; Ashkan Golshani; Myron L Smith Journal: Int J Food Microbiol Date: 2013-04-06 Impact factor: 5.277
Authors: Natalya N Besednova; Tatyana S Zaporozhets; Boris G Andryukov; Sergey P Kryzhanovsky; Svetlana P Ermakova; Tatyana A Kuznetsova; Anastasia N Voronova; Mikhail Y Shchelkanov Journal: Mar Drugs Date: 2021-11-12 Impact factor: 5.118