Chen Xin1, Shaozhen Song2, Ningli Wang1, Ruikang Wang2, Murray Johnstone3. 1. Beijing Tongren Eye Center, Beijing Institute of Ophthalmology, Beijing Tongren Hospital, Capital Medical University, Beijing 100730, China. 2. Department of Bioengineering, University of Washington, Seattle, WA 98195, USA. 3. Department of Ophthalmology, University of Washington, Seattle, WA 98195, USA.
Abstract
OBJECTIVE: To evaluate the change of biomechanical properties of the trabecular meshwork (TM) and configuration of collector channels (CC) by high-resolution optical coherence tomography (HR-OCT) induced by Schlemm's canal (SC) dilation. METHODS: The anterior segments of two human eyes were divided into four quadrants. One end of a specially designed cannula was placed in SC and the other end connected to a perfusion reservoir. HR-OCT provided three-dimensional (3D) volumetric and two-dimensional (2D) cross-sectional imaging permitting assessment of the biomechanical properties of the TM. A large fluid bolus was introduced into SC. Same-sample, pre and post deformation and disruption of SC and CC lumen areas were analyzed. RESULTS: Morphologic 3D reconstructions documented pressure-dependent changes in lumen dimension of SC, CC, and circumferential intrascleral channels. 2D imaging established volumetric stress-strain curves (elastance curves) of the TM in quadrants. The curves of TM elastance shift to the right with an increase in pressure-dependent steady-state SC area. After a bolus disruption, the SC area increased, while the CC area decreased. CONCLUSION: Our experimental setup permits the study of the biomechanical properties of TM by examining elastance, which differs segmentally and is altered by mechanical expansion of SC by a fluid bolus. The study may shed light on mechanisms of intraocular pressure control of some glaucoma surgery.
OBJECTIVE: To evaluate the change of biomechanical properties of the trabecular meshwork (TM) and configuration of collector channels (CC) by high-resolution optical coherence tomography (HR-OCT) induced by Schlemm's canal (SC) dilation. METHODS: The anterior segments of two human eyes were divided into four quadrants. One end of a specially designed cannula was placed in SC and the other end connected to a perfusion reservoir. HR-OCT provided three-dimensional (3D) volumetric and two-dimensional (2D) cross-sectional imaging permitting assessment of the biomechanical properties of the TM. A large fluid bolus was introduced into SC. Same-sample, pre and post deformation and disruption of SC and CC lumen areas were analyzed. RESULTS: Morphologic 3D reconstructions documented pressure-dependent changes in lumen dimension of SC, CC, and circumferential intrascleral channels. 2D imaging established volumetric stress-strain curves (elastance curves) of the TM in quadrants. The curves of TM elastance shift to the right with an increase in pressure-dependent steady-state SC area. After a bolus disruption, the SC area increased, while the CC area decreased. CONCLUSION: Our experimental setup permits the study of the biomechanical properties of TM by examining elastance, which differs segmentally and is altered by mechanical expansion of SC by a fluid bolus. The study may shed light on mechanisms of intraocular pressure control of some glaucoma surgery.
Authors: Murray Johnstone; Chen Xin; Ted Acott; Janice Vranka; Joanne Wen; Elizabeth Martin; Ruikang K Wang Journal: Front Med (Lausanne) Date: 2022-04-29
Authors: Xuan Wu; Bingyao Tan; Jinyuan Gan; Adeline R Lam; Yibing Chen; Xinyu Liu; Jacqueline Chua; Damon W K Wong; Marcus Ang; Leopold Schmetterer; Xinwen Yao Journal: Sci Rep Date: 2022-01-26 Impact factor: 4.379