Fawad Ahmad1, Muneera D F Alkahtani2, Muhammad Babar Taj3,4, Afnan M Alnajeebi5, Seraj Omar Alzahrani6, Nouf Abubakr Babteen5, Walla Alelwani5, Azzah M Bannunah7, Sadia Noor8, Rabia Ayub9, Syed Ahmad Tirmizi1, Heba Alshater10. 1. Department of Chemistry, Quaid-e-Azam University Islamabad, Islamabad 44000, Pakistan. 2. Department of Biology, College of Science, Princess Nourah Bint Abdulrahman University, Riyadh 11675, Saudi Arabia. 3. Department of Chemistry, Islamia University of Bahawalpur, Bahawalpur 63100, Pakistan. 4. Department of Chemistry, University of Sahiwal, Sahiwal 57000, Pakistan. 5. Department of Biochemistry, Faculty of Science, University of Jeddah, Jeddah 80203, Saudi Arabia. 6. Department of Chemistry, College of Science, Taibah University, Madinah 42353, Saudi Arabia. 7. Department of Basic Sciences, Common First Year Deanship, Umm Al-Qura University, Makkah 21955, Saudi Arabia. 8. Department of Chemistry, Govt. College for Women University Faisalabad, Faisalabad 38000, Pakistan. 9. Arrhenius Laboratory, Department of Organic Chemistry, Stockholm University, Svante Arrhenius Vag 16C, SE-10691 Stockholm, Sweden. 10. Department of Forensic Medicine and Clinical Toxicology, Menoufia University, Shbien El-Kom 32511, Egypt.
Abstract
In the present study, naphthyl acetohydrazide (HL) ligand was prepared and used for the synthesis of new six amorphous transition metal (Co(II), Ni(II), Cu(II), Zn(II), Pb(II), Cd(II)) complexes. All the compounds were characterized by elemental analysis, UV-vis, FT-IR, 1H- and 13C-NMR, and Matrix-Assisted Laser Desorption Ionization (MALDI). The solubilization study was carried out by estimating the interaction between the metal complexes with surfactants viz. sodium stearate (SS) and Cetyltrimethylammonium bromide (CTAB). UV-Visible spectroscopy was employed to determine partitioning and binding parameters, whereas electrical conductivity measurements were employed to estimate critical micellar concentration (CMC), the extent of dissociation, and free energy of micellization. The CT-DNA interaction of synthesized compounds with DNA represents the major groove binding. The synthesized ligand and metal complexes were also tested against bacterial and fungal strains and it has been observed that Cu(II) complex is active against all the strains except Candida albicans, while Cd(II) complex is active against all bacterial and fungal strains except Pseudomonas. Among all compounds, only the Pd(II) complex shows reasonable activity against cervical cancer HeLa cell lines, representing 97% inhibition.
In the present study, naphthyl acetohydrazide (n class="Gene">HL) ligand was prepared and used for the synthesis of new six amorphous transition metal (Co(II), Ni(II), Cu(II), Zn(II), Pb(II), Cd(II)) complexes. All the compounds were characterized by elemental analysis, UV-vis, FT-IR, 1H- and 13C-NMR, and Matrix-Assisted Laser Desorption Ionization (MALDI). The solubilization study was carried out by estimating the interaction between the metalcomplexes with surfactants viz. sodium stearate (SS) and Cetyltrimethylammonium bromide (CTAB). UV-Visible spectroscopy was employed to determine partitioning and binding parameters, whereas electrical conductivity measurements were employed to estimate critical micellar concentration (CMC), the extent of dissociation, and free energy of micellization. The CT-DNA interaction of synthesized compounds with DNA represents the major groove binding. The synthesized ligand and metalcomplexes were also tested against bacterial and fungal strains and it has been observed that Cu(II)complex is active against all the strains except Candida albicans, while Cd(II)complex is active against all bacterial and fungal strains except Pseudomonas. Among all compounds, only the Pd(II)complex shows reasonable activity against cervical cancerHeLa cell lines, representing 97% inhibition.
Authors: Olga Krasnovskaya; Alexey Naumov; Dmitry Guk; Peter Gorelkin; Alexander Erofeev; Elena Beloglazkina; Alexander Majouga Journal: Int J Mol Sci Date: 2020-05-31 Impact factor: 5.923