Literature DB >> 3366753

A cloned rat thymic epithelial cell line established from serum-free selective culture.

A Piltch1, P Naylor, J Hayashi.   

Abstract

A serum-free system has been developed for selective growth and long-term culture of rat thymic epithelial cells. The growth media is a modification of McKeehan's WAJC 404, plus insulin, cholera toxin, dexamethasone, and epidermal growth factor. Cultures have been continuously passaged and maintained for over 6 mo., and a cloned cell line, TEA3A1, has been established. These cells are epithelial, judging by morphology and ultrastructure, and are positive for A2B5 and thymosin alpha 1 markers for thymic endocrine cells.

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Year:  1988        PMID： 3366753     DOI： 10.1007/bf02628829

Source DB:  PubMed          Journal:  In Vitro Cell Dev Biol        ISSN： 0883-8364


  26 in total

1.  Establishment and characterization of a thymic medullary epithelial cell clone.

Authors:  E F Potworowski; F Turcotte; C Beauchemin; P Hugo; M G Zelechowska
Journal:  In Vitro Cell Dev Biol       Date:  1986-09

2.  Identification of Ia glycoproteins in rat thymus and purification from rat spleen.

Authors:  W R McMaster; A F Williams
Journal:  Eur J Immunol       Date:  1979-06       Impact factor: 5.532

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Authors:  R V Rouse; W van Ewijk; P P Jones; I L Weissman
Journal:  J Immunol       Date:  1979-06       Impact factor: 5.422

4.  Morphological and functional maturation of human thymic epithelium in culture.

Authors:  K W Pyke; E W Gelfand
Journal:  Nature       Date:  1974-10-04       Impact factor: 49.962

5.  The human thymic microenvironment: cortical thymic epithelium is an antigenically distinct region of the thymic microenvironment.

Authors:  E J McFarland; R M Scearce; B F Haynes
Journal:  J Immunol       Date:  1984-09       Impact factor: 5.422

6.  Thymic nurse cells--Ia-bearing epithelium involved in T-lymphocyte differentiation?

Authors:  H Wekerle; U P Ketelsen
Journal:  Nature       Date:  1980-01-24       Impact factor: 49.962

7.  Immunochemical studies on thymosin: radioimmunoassay of thymosin alpha 1.

Authors:  J E McClure; N Lameris; D W Wara; A L Goldstein
Journal:  J Immunol       Date:  1982-01       Impact factor: 5.422

8.  Long-term proliferation of mouse thymic epithelial cells in culture.

Authors:  U K Ehmann; R A Shiurba; W D Peterson
Journal:  In Vitro Cell Dev Biol       Date:  1986-12

9.  Identification of human and rodent thymic epithelium using tetanus toxin and monoclonal antibody A2B5.

Authors:  B F Haynes; K Shimizu; G S Eisenbarth
Journal:  J Clin Invest       Date:  1983-01       Impact factor: 14.808

10.  Macrophage and lymphocyte-depleted thymus reticuloepithelial cell cultures: establishment and functional influence on T-lymphocyte maturation, C-type virus expression and lymphomatous transformation in vitro.

Authors:  J Boniver; A Declève; M O Dailey; C Honsik; M Lieberman; H S Kaplan
Journal:  Thymus       Date:  1981-02
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  10 in total

1.  Development and characterization of a continuous macrophage cell line, LRTM, derived from thymus of Labeo rohita (Hamilton 1822).

Authors:  Sanjay C Rebello; Gaurav Rathore; Peyush Punia; Neeraj Sood; V Elangovan
Journal:  In Vitro Cell Dev Biol Anim       Date:  2013-08-17       Impact factor: 2.416

2.  Establishment of functional epithelial cell lines from a rat thymoma and a rat thymus.

Authors:  A Masuda; K Ohtsuka; M Matsuyama
Journal:  In Vitro Cell Dev Biol       Date:  1990-07

3.  Cellular levels of thymosin immunoreactive peptides are linked to proliferative events: evidence for a nuclear site of action.

Authors:  C N Conteas; M G Mutchnick; K C Palmer; F E Weller; G D Luk; P H Naylor; M R Erdos; A L Goldstein; C Panneerselvam; B L Horecker
Journal:  Proc Natl Acad Sci U S A       Date:  1990-05       Impact factor: 11.205

4.  Thymic nurse cells in culture: morphological and antigenic characterization.

Authors:  D Toussaint-Demylle; J M Scheiff; S Haumont
Journal:  Cell Tissue Res       Date:  1993-05       Impact factor: 5.249

5.  Short-term cultivation of murine thymic epithelial cells in a serum-free medium.

Authors:  C Ropke; B van Deurs; O W Petersen
Journal:  In Vitro Cell Dev Biol       Date:  1990-07

6.  Activation of phospholipase A2 and stimulation of prostaglandin E2 production by transforming growth factor-alpha in rat thymic epithelial cells requires influx of calcium.

Authors:  P Liu; M Wen; L Sun; J Hayashi
Journal:  Biochem J       Date:  1993-07-01       Impact factor: 3.857

7.  Cloning and characterization of Lnk, a signal transduction protein that links T-cell receptor activation signal to phospholipase C gamma 1, Grb2, and phosphatidylinositol 3-kinase.

Authors:  X Huang; Y Li; K Tanaka; K G Moore; J I Hayashi
Journal:  Proc Natl Acad Sci U S A       Date:  1995-12-05       Impact factor: 11.205

8.  Lipocortin-independent effect of dexamethasone on phospholipase activity in a thymic epithelial cell line.

Authors:  A Piltch; L Sun; R A Fava; J Hayashi
Journal:  Biochem J       Date:  1989-07-15       Impact factor: 3.857

9.  Characterization of ATP receptor responsible for the activation of phospholipase A2 and stimulation of prostaglandin E2 production in thymic epithelial cells.

Authors:  P Liu; M Wen; J Hayashi
Journal:  Biochem J       Date:  1995-06-01       Impact factor: 3.857

10.  Identification of a thymic epithelial cell subset sharing expression of the class Ib HLA-G molecule with fetal trophoblasts.

Authors:  L Crisa; M T McMaster; J K Ishii; S J Fisher; D R Salomon
Journal:  J Exp Med       Date:  1997-07-21       Impact factor: 14.307
  10 in total

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