Strontium as a tracer to study the transport of calcium in the epiphyseal growth plate (electronprobe microanalysis).

At the mineralization front of the epiphyseal growth plate large quantities of calcium (Ca) are necessary to form the mineral (a Ca-phosphate). It is an unsolved problem, whether Ca is transported through the cells of the growth plate cartilage or extracellularly. Electronprobe microanalysis (EPMA) allows the quantitative, morphologically correlated analysis of elements. EPMA can discriminate only different elements. To investigate the transport of Ca, Strontium (Sr) is a very good tracer, as it resembles Ca in many biological reactions. Our results demonstrate that the transport of Sr from the blood into the growth plate and through the growth plate needs only one or a few minutes. The measured intracellular Sr and Ca concentrations are much lower than the extracellular ones, while the intracellular Sr/Ca ratio is not or only a little bit lower than the extracellular one. It must be concluded, that significant amounts of Ca are neither transported through nor accumulated in the cells of the growth plate cartilage. The main transport is an extracellular diffusion. Using Sr as a tracer for Ca new results on the behaviour of Ca could be received.