Hajar Khazraei1, Seyedeh Azra Shamsdin2, Mozhdeh Zamani3,4. 1. Colorectal Research Center, Shiraz University of Medical Sciences, Shiraz, Iran. 2. Gastroenterohepatology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran. 3. Colorectal Research Center, Shiraz University of Medical Sciences, Shiraz, Iran. mozamani@sums.ac.ir. 4. Autophagy Research Center, Shiraz University of Medical Sciences, Shiraz, Iran. mozamani@sums.ac.ir.
Abstract
PURPOSE: Toxic effects of vegetables have been the subject of numerous investigations in recent years. Eucalyptus globulus of the Myrtaceae family has been traditionally used in the treatment of various infectious disorders. This study aims to assay the potential anticancer effect of Eucalyptus globulus essential oil, in vitro, and compare its cytotoxic effects on cancerous and non-cancerous cell lines. METHODS: The cytotoxicity of the Eucalyptus globulus essential oil was evaluated in colon cancer cell line SW48, liver cancer cell line HepG2, HEK293t, and skin fibroblast using the MTT assay. We used 5-FU as the control treatment for cancer. The essential oil was diluted by phosphate buffer saline in cell culture. RESULTS: Eucalyptus globulus essential oil with the concentration of 0.01% did not reduce the cell viability of SW48 (IC50 = 0.2%) and fibroblast cells (IC50 = 5%) after a 48-h treatment period. Eucalyptus globulus essential oil did not also exhibit any toxic effects on HEK293t and HEPG2 cell lines, except in high dose (0.5%) with IC50 = 0.2% for both of the cell lines. CONCLUSION: Eucalyptus essential oil showed some side effects and might not be safe to use for treatment at high doses (0.5% and 5%). Moreover, the findings of the present study show that eucalyptus essential oil suppressed the proliferation of human colon cancer cells by inducing apoptosis. Eucalyptus essential oil might be a good candidate for the treatment of colon cancer.
PURPOSE: Toxic effects of vegetables have been the subject of numerous investigations in recent years. Eucalyptus globulus of the Myrtaceae family has been traditionally used in the treatment of various infectious disorders. This study aims to assay the potential anticancer effect of Eucalyptus globulus essential oil, in vitro, and compare its cytotoxic effects on cancerous and non-cancerous cell lines. METHODS: The cytotoxicity of the Eucalyptus globulus essential oil was evaluated in colon cancer cell line SW48, liver cancer cell line HepG2, HEK293t, and skin fibroblast using the MTT assay. We used 5-FU as the control treatment for cancer. The essential oil was diluted by phosphate buffer saline in cell culture. RESULTS: Eucalyptus globulus essential oil with the concentration of 0.01% did not reduce the cell viability of SW48 (IC50 = 0.2%) and fibroblast cells (IC50 = 5%) after a 48-h treatment period. Eucalyptus globulus essential oil did not also exhibit any toxic effects on HEK293t and HEPG2 cell lines, except in high dose (0.5%) with IC50 = 0.2% for both of the cell lines. CONCLUSION: Eucalyptus essential oil showed some side effects and might not be safe to use for treatment at high doses (0.5% and 5%). Moreover, the findings of the present study show that eucalyptus essential oil suppressed the proliferation of human colon cancer cells by inducing apoptosis. Eucalyptus essential oil might be a good candidate for the treatment of colon cancer.
Authors: Dominik Radzki; Marta Wilhelm-Węglarz; Katarzyna Pruska; Aida Kusiak; Iwona Ordyniec-Kwaśnica Journal: Int J Environ Res Public Health Date: 2022-03-25 Impact factor: 3.390
Authors: Khaled A Nematallah; Sahar Elmekkawy; Maha R A Abdollah; Mohey M Elmazar; Essam Abdel-Sattar; Meselhy Ragab Meselhy Journal: ACS Omega Date: 2022-02-24