Literature DB >> 336601

Selection of yeast auxotrophs by thymidylate starvation.

B J Barclay, J G Little.   

Abstract

A rapid procedure for the recovery of Saccharomyces cerevisiae auxotrophs was developed by exploiting the protection of these mutants from thymineless death when a required metabolite was withheld. The method can be used for thymidine 5'-monophosphate-requiring auxotrophs or wild-type strains blocked in de novo synthesis of thymidylate by folate antagonists.

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Year:  1977        PMID： 336601      PMCID： PMC235609          DOI： 10.1128/jb.132.3.1036-1037.1977

Source DB:  PubMed          Journal:  J Bacteriol        ISSN： 0021-9193            Impact factor:   3.490


  1 in total

1.  Selection of spontaneous mutants by inositol starvation in yeast.

Authors:  S A Henry; T F Donahue; M R Culbertson
Journal:  Mol Gen Genet       Date:  1975-12-30
  1 in total
  8 in total

1.  Enrichment for auxotrophic mutants in Saccharomyces cerevisiae using the cell wall inhibitor, echinocandin B.

Authors:  M T McCammon; L W Parks
Journal:  Mol Gen Genet       Date:  1982

2.  Selection and fusion of auxotrophic protoplasts of Candida albicans.

Authors:  K O Evans; A Adeniji; D O McClary
Journal:  Antonie Van Leeuwenhoek       Date:  1982-05       Impact factor: 2.271

3.  Cytocidal amino acid starvation of Saccharomyces cerevisiae and Candida albicans acetolactate synthase (ilv2{Delta}) mutants is influenced by the carbon source and rapamycin.

Authors:  Joanne M Kingsbury; John H McCusker
Journal:  Microbiology (Reading)       Date:  2009-12-17       Impact factor: 2.777

4.  Enhanced canavanine uptake is associated with nucleotide permeability in a thymidylate auxotroph of Saccharomyces cerevisiae.

Authors:  S E Kohalmi; B A Kunz
Journal:  Curr Genet       Date:  1989-02       Impact factor: 3.886

5.  Induction of mitotic recombination in yeast by starvation for thymine nucleotides.

Authors:  B A Kunz; B J Barclay; J C Game; J G Little; R H Haynes
Journal:  Proc Natl Acad Sci U S A       Date:  1980-10       Impact factor: 11.205

6.  Growth inhibition of Candida albicans by folate pathway inhibitors. Their potential in the selection of auxotrophs.

Authors:  O E Henson; D O McClary
Journal:  Antonie Van Leeuwenhoek       Date:  1979       Impact factor: 2.271

7.  Parasexual genetic analysis of Candida albicans by spheroplast fusion.

Authors:  R Poulter; K Jeffery; M J Hubbard; M G Shepherd; P A Sullivan
Journal:  J Bacteriol       Date:  1981-06       Impact factor: 3.490

8.  dUTP pyrophosphatase is an essential enzyme in Saccharomyces cerevisiae.

Authors:  M H Gadsden; E M McIntosh; J C Game; P J Wilson; R H Haynes
Journal:  EMBO J       Date:  1993-11       Impact factor: 11.598
  8 in total

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