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Literature DB >> 336599

Reconstitution and purification of flagellar filaments from Caulobacter crescentus.

Abstract

Filaments from isolated flagella of Caulobacter crescentus have been purified by successive dissociation and reconstitution. After the second and third reconstitutions from subunits in 0.8 M sodium citrate, filament preparations contained only two proteins, flagellin A (26,000 daltons) and flagellin B (28,000 daltons). There was some enrichment for flagellin A during reconstitution by this procedure, since isolated flagella contained flagellin A and flagellin B in a ratio of approximately 3.8:1 and filaments after the third reconstitution contained the two proteins in a ratio of 5.0:1.

Entities: Chemical Species

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Year: 1977 PMID： 336599 PMCID： PMC235606 DOI： 10.1128/jb.132.3.1027-1030.1977

Source DB: PubMed Journal: J Bacteriol ISSN： 0021-9193 Impact factor: 3.490

12 in total

1. IN VITRO FORMATION OF FLAGELLA-LIKE FILAMENTS AND OTHER STRUCTURES FROM FLAGELLIN.

Authors: D ABRAM; H KOFFLER
Journal: J Mol Biol Date: 1964-07 Impact factor: 5.469

2. BIOLOGICAL PROPERTIES AND CLASSIFICATION OF THE CAULOBACTER GROUP.

Authors: J S POINDEXTER
Journal: Bacteriol Rev Date: 1964-09

3. Protein measurement with the Folin phenol reagent.

Authors: O H LOWRY; N J ROSEBROUGH; A L FARR; R J RANDALL
Journal: J Biol Chem Date: 1951-11 Impact factor: 5.157

4. Regulation of flagellin synthesis in the cell cycle of caulobacter: dependence on DNA replication.

Authors: M A Osley; M Sheffery; A Newton
Journal: Cell Date: 1977-10 Impact factor: 41.582

5. Mutational analysis of developmental control in Caulobacter crescentus.

Authors: M A Osley; A Newton
Journal: Proc Natl Acad Sci U S A Date: 1977-01 Impact factor: 11.205

Review 6. Bacterial flagella.

Authors: R W Smith; H Koffler
Journal: Adv Microb Physiol Date: 1971 Impact factor: 3.517

Review 7. Genetics and chemistry of bacterial flagella.

Authors: T Iino
Journal: Bacteriol Rev Date: 1969-12

8. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors: U K Laemmli
Journal: Nature Date: 1970-08-15 Impact factor: 49.962

9. Physical characterization of Caulobacter crescentus flagella.

Authors: C Lagenaur; N Agabian
Journal: J Bacteriol Date: 1976-10 Impact factor: 3.490

10. Purification of intact flagella from Escherichia coli and Bacillus subtilis.

Authors: M L DePamphilis; J Adler
Journal: J Bacteriol Date: 1971-01 Impact factor: 3.490

15 in total

1. A family of six flagellin genes contributes to the Caulobacter crescentus flagellar filament.

Authors: B Ely; T W Ely; W B Crymes; S A Minnich
Journal: J Bacteriol Date: 2000-09 Impact factor: 3.490

2. Molecular genetics of the flgI region and its role in flagellum biosynthesis in Caulobacter crescentus.

Authors: F M Khambaty; B Ely
Journal: J Bacteriol Date: 1992-06 Impact factor: 3.490

Review 3. Getting in the loop: regulation of development in Caulobacter crescentus.

Authors: Patrick D Curtis; Yves V Brun
Journal: Microbiol Mol Biol Rev Date: 2010-03 Impact factor: 11.056

4. Analysis of nonmotile mutants of the dimorphic bacterium Caulobacter crescentus.

Authors: R C Johnson; B Ely
Journal: J Bacteriol Date: 1979-01 Impact factor: 3.490

5. Evidence that subcellular flagellin pools in Caulobacter crescentus are precursors in flagellum assembly.

Authors: E D Huguenel; A Newton
Journal: J Bacteriol Date: 1984-03 Impact factor: 3.490

6. [Immunological and electrophoretic characterization of flagellins of different H-types of Pseudomonas aeruginosa (author's transl)].

Authors: R Ansorg; W Schmitt
Journal: Med Microbiol Immunol Date: 1980 Impact factor: 3.402

10. Isolation of flagellated membrane vesicles from Caulobacter crescentus cells: evidence for functional differentiation of polar membrane domains.

Authors: E Huguenel; A Newton
Journal: Proc Natl Acad Sci U S A Date: 1984-06 Impact factor: 11.205