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Literature DB >> 33659381

In-vitro GLP-1 Release Assay Using STC-1 Cells.

Liu Qi¹, Tan Shuai¹, Jia Da², Burstein Ezra¹, Sifuentes-Dominguez Luis³.

Abstract

Enteroendocrine cells (EECs) are known chemosensors in the gastrointestinal (GI) epithelium. They release a diversity of gut hormones in response to various stimuli. Here, we report an in-vitro assay to measure GLP-1 release from cultured murine EEC's under fatty acid stimulation.

Entities: Chemical

Keywords: Docosahexaenoic acid (DHA); Enteroendocrine cells; Glucagon-like peptide-1 (GLP-1); L-cells; STC-1

Year: 2020 PMID： 33659381 PMCID： PMC7854363 DOI： 10.21769/BioProtoc.3717

Source DB: PubMed Journal: Bio Protoc ISSN： 2331-8325

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References

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In-vitro GLP-1 Release Assay Using STC-1 Cells.

1. The gut as a sensory organ.

2. Enteroendocrine L Cells Sense LPS after Gut Barrier Injury to Enhance GLP-1 Secretion.

3. Development of neuroendocrine tumors in the gastrointestinal tract of transgenic mice. Heterogeneity of hormone expression.

4. Fatty acid-induced cholecystokinin secretion and changes in intracellular Ca2+ in two enteroendocrine cell lines, STC-1 and GLUTag.

Review 5. The incretin system: glucagon-like peptide-1 receptor agonists and dipeptidyl peptidase-4 inhibitors in type 2 diabetes.

6. Fatty acids stimulate cholecystokinin secretion via an acyl chain length-specific, Ca2+-dependent mechanism in the enteroendocrine cell line STC-1.

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