Literature DB >> 33659374

High-level Production of Recombinant Membrane Proteins Using the Engineered Escherichia coli Strains SuptoxD and SuptoxR.

Myrsini Michou1,2, Dafni C Delivoria1, Georgios Skretas1.   

Abstract

We have previously described the development of two specialized Escherichia coli strains for high-level recombinant membrane protein (MP) production. These engineered strains, termed SuptoxD and SuptoxR, are capable of suppressing the cytotoxicity caused by MP overexpression and of producing greatly enhanced MP yields. Here, we present a Bio-protocol that describes gene overexpression and culturing conditions that maximize the accumulation of membrane-integrated and well-folded recombinant MPs in these strains.
Copyright © 2020 The Authors; exclusive licensee Bio-protocol LLC.

Entities:  

Keywords:  Escherichia coli; Membrane protein; Recombinant protein production; SuptoxD; SuptoxR; Toxicity

Year:  2020        PMID： 33659374      PMCID： PMC7842518          DOI： 10.21769/BioProtoc.3710

Source DB:  PubMed          Journal:  Bio Protoc        ISSN： 2331-8325


  18 in total

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Authors:  Samuel Wagner; Mirjam Lerch Bader; David Drew; Jan-Willem de Gier
Journal:  Trends Biotechnol       Date:  2006-07-03       Impact factor: 19.536

2.  Efficient production of membrane-integrated and detergent-soluble G protein-coupled receptors in Escherichia coli.

Authors:  A James Link; Georgios Skretas; Eva-Maria Strauch; Nandini S Chari; George Georgiou
Journal:  Protein Sci       Date:  2008-07-01       Impact factor: 6.725

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Authors:  Muhammed A Yildirim; Kwang-Il Goh; Michael E Cusick; Albert-László Barabási; Marc Vidal
Journal:  Nat Biotechnol       Date:  2007-10       Impact factor: 54.908

4.  Functional Requirements for DjlA- and RraA-Mediated Enhancement of Recombinant Membrane Protein Production in the Engineered Escherichia coli Strains SuptoxD and SuptoxR.

Authors:  Dimitra Gialama; Dafni Chrysanthi Delivoria; Myrsini Michou; Artemis Giannakopoulou; Georgios Skretas
Journal:  J Mol Biol       Date:  2017-05-10       Impact factor: 5.469

Review 5.  Microbial expression systems for membrane proteins.

Authors:  Marvin V Dilworth; Mathilde S Piel; Kim E Bettaney; Pikyee Ma; Ji Luo; David Sharples; David R Poyner; Stephane R Gross; Karine Moncoq; Peter J F Henderson; Bruno Miroux; Roslyn M Bill
Journal:  Methods       Date:  2018-04-12       Impact factor: 3.608

6.  Development of Escherichia coli Strains That Withstand Membrane Protein-Induced Toxicity and Achieve High-Level Recombinant Membrane Protein Production.

Authors:  Dimitra Gialama; Kalliopi Kostelidou; Myrsini Michou; Dafni Chrysanthi Delivoria; Fragiskos N Kolisis; Georgios Skretas
Journal:  ACS Synth Biol       Date:  2016-11-17       Impact factor: 5.110

Review 7.  Over-production of proteins in Escherichia coli: mutant hosts that allow synthesis of some membrane proteins and globular proteins at high levels.

Authors:  B Miroux; J E Walker
Journal:  J Mol Biol       Date:  1996-07-19       Impact factor: 5.469

8.  Optimization of Recombinant Membrane Protein Production in the Engineered Escherichia coli Strains SuptoxD and SuptoxR.

Authors:  Myrsini Michou; Charalampos Kapsalis; Christos Pliotas; Georgios Skretas
Journal:  ACS Synth Biol       Date:  2019-06-25       Impact factor: 5.110

9.  Use of the tetracycline promoter for the tightly regulated production of a murine antibody fragment in Escherichia coli.

Authors:  A Skerra
Journal:  Gene       Date:  1994-12-30       Impact factor: 3.688

10.  Consequences of membrane protein overexpression in Escherichia coli.

Authors:  Samuel Wagner; Louise Baars; A Jimmy Ytterberg; Anja Klussmeier; Claudia S Wagner; Olof Nord; Per-Ake Nygren; Klaas J van Wijk; Jan-Willem de Gier
Journal:  Mol Cell Proteomics       Date:  2007-04-19       Impact factor: 5.911
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  1 in total

1.  Second-Generation Escherichia coli SuptoxR Strains for High-Level Recombinant Membrane Protein Production.

Authors:  Eleni Vasilopoulou; Artemis Giannakopoulou; Charalampos Kapsalis; Myrsini Michou; Aristeidis Michoglou-Sergiou; Fragiskos N Kolisis; Georgios Skretas
Journal:  ACS Synth Biol       Date:  2022-08-03       Impact factor: 5.249
  1 in total

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