Literature DB >> 33659340

Isolation of Lipid Rafts from Cultured Mammalian Cells and Their Lipidomics Analysis.

Nigora Mukhamedova1, Kevin Huynh1, Hann Low1, Peter J Meikle1, Dmitri Sviridov1.   

Abstract

Lipid rafts are distinct liquid-ordered domains of plasma membranes of most eukaryotic cells providing platform for signaling pathways. Lipid composition of rafts is critical for their structural integrity and for regulation of signaling pathways originating from rafts. Here we provide a protocol to isolate lipid rafts from cultured human and animal cells and comprehensively analyse their lipid composition.
Copyright © 2020 The Authors; exclusive licensee Bio-protocol LLC.

Entities:  

Keywords:  Cholesterol; Lipid rafts; Lipidomics; Lipids; Membrane

Year:  2020        PMID： 33659340      PMCID： PMC7842516          DOI： 10.21769/BioProtoc.3670

Source DB:  PubMed          Journal:  Bio Protoc        ISSN： 2331-8325


  6 in total

1.  High-Throughput Plasma Lipidomics: Detailed Mapping of the Associations with Cardiometabolic Risk Factors.

Authors:  Kevin Huynh; Christopher K Barlow; Kaushala S Jayawardana; Jacquelyn M Weir; Natalie A Mellett; Michelle Cinel; Dianna J Magliano; Jonathan E Shaw; Brian G Drew; Peter J Meikle
Journal:  Cell Chem Biol       Date:  2018-11-08       Impact factor: 8.116

2.  Domain-specific lipid distribution in macrophage plasma membranes.

Authors:  Katharina Gaus; Macarena Rodriguez; Kalani R Ruberu; Ingrid Gelissen; Timothy M Sloane; Leonard Kritharides; Wendy Jessup
Journal:  J Lipid Res       Date:  2005-05-01       Impact factor: 5.922

3.  Cholesterol efflux assay.

Authors:  Hann Low; Anh Hoang; Dmitri Sviridov
Journal:  J Vis Exp       Date:  2012-03-06       Impact factor: 1.355

4.  Plasma lipid profiling in a large population-based cohort.

Authors:  Jacquelyn M Weir; Gerard Wong; Christopher K Barlow; Melissa A Greeve; Adam Kowalczyk; Laura Almasy; Anthony G Comuzzie; Michael C Mahaney; Jeremy B M Jowett; Jonathan Shaw; Joanne E Curran; John Blangero; Peter J Meikle
Journal:  J Lipid Res       Date:  2013-07-18       Impact factor: 5.922

Review 5.  Lipid rafts as a membrane-organizing principle.

Authors:  Daniel Lingwood; Kai Simons
Journal:  Science       Date:  2010-01-01       Impact factor: 47.728

6.  Exosomes containing HIV protein Nef reorganize lipid rafts potentiating inflammatory response in bystander cells.

Authors:  Nigora Mukhamedova; Anh Hoang; Dragana Dragoljevic; Larisa Dubrovsky; Tatiana Pushkarsky; Hann Low; Michael Ditiatkovski; Ying Fu; Ryunosuke Ohkawa; Peter J Meikle; Anelia Horvath; Beda Brichacek; Yury I Miller; Andrew Murphy; Michael Bukrinsky; Dmitri Sviridov
Journal:  PLoS Pathog       Date:  2019-07-25       Impact factor: 6.823
  6 in total
  1 in total

1.  Amyloidogenic, neuroinflammatory and memory dysfunction effects of HIV-1 gp120.

Authors:  Young-Jung Lee; In Jun Yeo; Dong Young Choi; Jaesuk Yun; Dong Ju Son; Sang-Bae Han; Jin Tae Hong
Journal:  Arch Pharm Res       Date:  2021-07-23       Impact factor: 4.946
  1 in total

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