Literature DB >> 33659309

Ex vivo Culture Assay Using Human Hair Follicles to Study Circadian Characteristics.

Atsuhiro Nishida1, Yoshiki Miyawaki1, Koichi Node2, Makoto Akashi1.   

Abstract

Ex vivo culture assays of biopsy specimens are advantageous for the experimental evaluation of human circadian characteristics. We developed a simple and non-invasive experimental evaluation method for monitoring the expression of circadian clock genes in an ex vivo culture assay using human hair follicles. This method imposes little burden on subjects. This assay is useful for validating correlations between circadian characteristics in hair follicles and intrinsic characteristics observed in physiological and behavioral studies. While they should be further validated, this ex vivo method constitutes a useful tool for estimating in vivo circadian characteristics.
Copyright © 2020 The Authors; exclusive licensee Bio-protocol LLC.

Entities:  

Keywords:  Circadian rhythm; Clock gene; Ex vivo; Hair follicle; Human; Non-invasive

Year:  2020        PMID： 33659309      PMCID： PMC7842322          DOI： 10.21769/BioProtoc.3638

Source DB:  PubMed          Journal:  Bio Protoc        ISSN： 2331-8325


  9 in total

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Journal:  Nat Rev Genet       Date:  2001-09       Impact factor: 53.242

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Authors:  Steven M Reppert; David R Weaver
Journal:  Nature       Date:  2002-08-29       Impact factor: 49.962

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Journal:  Cell       Date:  1999-01-22       Impact factor: 41.582

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Authors:  Takako Noguchi; Connie W Wang; Haiyun Pan; David K Welsh
Journal:  Chronobiol Int       Date:  2012-07       Impact factor: 2.877

5.  Extracellular low pH affects circadian rhythm expression in human primary fibroblasts.

Authors:  Sang Kil Lee; Elsie Achieng; Connie Maddox; Suephy C Chen; P Michael Iuvone; Chiaki Fukuhara
Journal:  Biochem Biophys Res Commun       Date:  2011-11-15       Impact factor: 3.575

6.  Comparison of the Munich Chronotype Questionnaire with the Horne-Ostberg's Morningness-Eveningness Score.

Authors:  Andrei Zavada; Marijke C M Gordijn; Domien G M Beersma; Serge Daan; Till Roenneberg
Journal:  Chronobiol Int       Date:  2005       Impact factor: 2.877

7.  In vitro circadian period is associated with circadian/sleep preference.

Authors:  Akiko Hida; Shingo Kitamura; Yosuke Ohsawa; Minori Enomoto; Yasuko Katayose; Yuki Motomura; Yoshiya Moriguchi; Kentaro Nozaki; Makiko Watanabe; Sayaka Aritake; Shigekazu Higuchi; Mie Kato; Yuichi Kamei; Shin Yamazaki; Yu-Ichi Goto; Masaaki Ikeda; Kazuo Mishima
Journal:  Sci Rep       Date:  2013       Impact factor: 4.379

8.  The period length of fibroblast circadian gene expression varies widely among human individuals.

Authors:  Steven A Brown; Fabienne Fleury-Olela; Emi Nagoshi; Conrad Hauser; Cristiana Juge; Christophe A Meier; Rachel Chicheportiche; Jean-Michel Dayer; Urs Albrecht; Ueli Schibler
Journal:  PLoS Biol       Date:  2005-09-27       Impact factor: 8.029

9.  A simple method using ex vivo culture of hair follicle tissue to investigate intrinsic circadian characteristics in humans.

Authors:  Ai Yamaguchi; Ritsuko Matsumura; Takashi Matsuzaki; Wataru Nakamura; Koichi Node; Makoto Akashi
Journal:  Sci Rep       Date:  2017-07-28       Impact factor: 4.379
  9 in total

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