The Analysis of Anxiety and Depression in Different Stages of in vitro Fertilization-Embryo Transfer in Couples in China.

BACKGROUND: This study aimed to examine the differences in anxiety and depression between infertile Chinese couples in diverse stages of in vitro fertilization-embryo transfer (IVF-ET) and their relationship with the IVF-ET outcomes.
METHODS: From February 2016 to December 2018, a total of 247 couples that were undergoing IVF-ET were randomly selected for this study. On the day they started their treatment (T1), the day human chorionic gonadotropin was administered (T2), and 4 days after the embryo transfer (T3), self-designed questionnaires, the Self-Rating Anxiety Scale, and the Self-Rating Depression Scale were completed to investigate anxiety and depression in different stages.
RESULTS: Age had an effect on the anxiety and depression in women. Male infertility type and the cause of infertility had an effect on the anxiety and depression in men. The incidence of anxiety in women in the T1, T2, and T3 stages was 29.96%, 44.94%, and 17.81%, respectively. The anxiety scores of women were 46.14 ± 8.37, 50.83 ± 8.50, and 44.09 ± 8.17, respectively, which were significantly higher than those of men (p < 0.05). The anxiety score in stage T2 was the highest in women, and the depression score of women in stage T1 was the highest. The incidence of anxiety in men in the T1, T2, and T3 stages was 20.65%, 8.50%, and 6.07%, respectively. The incidence of anxiety was not significantly different in diverse stages (p > 0.05), and the same result was obtained for the incidence of depression. The anxiety and depression scores of the infertile couples in different stages were not related to the outcome of IVF-ET.
CONCLUSION: The incidence of anxiety and depression in infertile couples in diverse stages of IVF-ET is different, especially in women, and the anxiety and depression of infertile couples in the process of IVF-ET may not be related to the outcome of assisted pregnancy.

Background

Infertile couples experience pressure from society, family, and treatment and have a higher risk of negative emotions, such as anxiety and depression.1–3 In vitro fertilization-embryo transfer (IVF-ET) is a method in which infertile couples participate to achieve pregnancy. This method has the problems of high cost and uncertain treatment effect, which may cause couples undergoing IVF-ET to experience great pressure. IVF-ET involves different stages, including ovulation induction, ovulation retrieval, and embryo transfer, and patients will face various difficulties in different stages. For example, patients in the stage of ovulation induction will worry about the effect of ovulation-promoting drugs, pain, the number of oocytes taken before ovulation, and whether the embryo can be implanted after transplantation. Whether the anxiety and depression of infertile couples in diverse stages of IVF-ET are different and whether they are related to the outcome of assisted pregnancy has been rarely reported. Chinese infertile couples may be affected by the traditional idea of succession, and they are under greater psychological pressure. Therefore, this study analyzed the anxiety, depression, and related factors of infertile couples in different stages of IVF-ET assisted pregnancy, as well as their association with the outcome of assisted pregnancy.

Patients

Between February 2016 and December 2018, couples undergoing IVF-ET in the Department of Reproductive Technology of the Affiliated Hospital of Southwest Medical University were reviewed on the first day of entering the cycle (T1), the day human chorionic gonadotropin (hCG) was administered (T2), and 4 days after the embryo transfer (T3). Informed consent was obtained from all the patients, and this study protocol was approved by the Ethics Committee of our hospital.

Inclusion criteria: 1) Women aged under 35 years; 2) women with an ovarian reserve function that was normal; 3) women participating in the regular luteal phase long protocol; and 4) women receiving initial IVF cycles.

Exclusion criteria: Patients had major psychological trauma in the past two years.

Methods

The questionnaire used to determine the couples’ basic condition was designed by the team prior to conducting the study. It included age, education level, infertility period, annual family income, and marital relationship.

The symptoms of anxiety were evaluated using the Zung Self-Rating Anxiety Scale (SAS).4 The SAS consisted of 20 items, and each item was answered on a 4-point Likert-type scale ranging from “never” to “always”. The standard score was the actual score multiplied by 1.25. The anxiety state was indicated by the SAS standard score ≥50. The degree of anxiety was classified as mild with a score of 50–59 (raw scores 40–47), moderate with a score of 60–69 (raw scores 48–55), and severe with a score of ≥70 (raw scores 56 and over).

The Zung Self-Rating Depression Scale (SDS) was used to assess depressive symptoms.5 The SDS is a 20-item self-report questionnaire, and it was used as a screening tool. Each item was scored from 1 to 4, and the total score was provided by adding the item scores. The standard score was the actual score multiplied by 1.25. The depression state was indicated by the SDS standard score ≥50. The degree of depression was classified as mild with a score of 50–59 (raw scores 40–47), moderate with a score of 60–69 (raw scores 48–55), and severe with a score of ≥70 (raw scores 56 and over). All the participants were informed of the purpose, content, and requirements of the survey to eliminate uncertainties and nervousness. The questionnaires were sent to the couples after they had signed the informed consent form, and all the questionnaires were anonymous. The questionnaire was completed on the first day of entering the cycle (T1), the day of hCG administration (T2), and 4 days after the embryo transfer (T3). All the women and men completed the questionnaire independently. For those with a low education level who could not complete the questionnaire independently, the doctor in charge of the questionnaire described the content of the questionnaire and the patient answered accordingly.

Statistical Analyses

Continuous variables were expressed as mean ± standard deviation (normal distribution) or median, while categorical data were expressed as n (%). All the data were analyzed with SPSS 21.0 (SPSS Inc., Chicago, IL, USA). The statistical methods included a chi-square test/Fisher’s exact test, independent sample t-test (continuous variables conforming to normal distribution by Shapiro–Wilk test), analysis of variance, and logistic regression analysis. A p-value of less than 0.05 was considered significant.

Results

The Influencing Factors of Anxiety and Depression in Women Receiving IVF-ET on the First Day of Entering the Cycle

A total of 247 couples were enrolled in this study (Figure 1). The average age of the women was 29.40 ± 2.98 years, the period of infertility was 3.1 ± 1.9 years, and the average age of the men was 31.72 ± 4.86 years.

Figure 1

Flow chart for study design.

In the 247 women receiving IVF-ET, 29.96% (74/247) had anxiety on the day of starting the treatment, and the percentage of those with mild, moderate, and severe anxiety was 20.65% (51/247), 7.69% (19/247), and 1.62% (4/247), respectively. Moreover, 15.79% (39/247) had depression, and the percentage of those with mild, moderate, and severe depression was 12.55% (31/247), 2.42% (6/247), and 0.81% (2/247), respectively. By analyzing the association between anxiety, depression, and their characteristics, we found that there were significant differences in the incidence of anxiety in women in terms of age, infertility period, types of infertility, education level, annual family income, family support, and marital relationship (p < 0.05). Furthermore, the incidence of depression was different according to the patients’ age, infertility period, education level, and marital relationship (p < 0.05) (Table 1). Logistic regression analysis showed that age, education level, and annual family income had a significant effect on anxiety in women receiving IVF-ET, while age and period of infertility had a significant impact on depression (p < 0.05, Table 2).

Table 1

Univariate Analysis of Anxiety and Depression-Related Factors in IVF-ET Women

Related Factors	Anxiety (n,%)		Χ2	P	Depression (n,%)		Χ2	P
	Yes	No			Yes	No
Age(y)			68.32	0.00			16.97	0.00
<30(126)	8(6.30)	118(93.70)			9(7.10)	117(92.90)
≥30(121)	66(54.50)	55(45.50)			30(27.00)	81(73.00)
Infertility period(y)			11.47	0.00			28.19	0.00
<5(134)	28(20.90)	106(79.10)			6(4.50)	128(95.50)
≥5(113)	46(40.70)	67(59.30)			33(29.20)	80(70.80)
Infertility type			4.74	0.00			0.07	0.47
Primary infertility(106)	24(22.60)	82(77.40)			16(15.10)	90(84.90)
Secondary infertility(141)	50(35.5)	91(64.5)			23(16.30)	118(83.70)
Cause of infertility			6.47	0.09			2.30	0.51
Female(118)	44(37.30)	74(62.70)			18(15.30)	100(84.70)
Male (47)	13(27.70)	34(72.30)			10(21.30)	37(78.70)
Both sides(62)	13(21.00)	49(79.00)			7(11.30)	55(88.70)
Unknown reason(20)	4(20.00)	16(80.00)			4(20.00)	16(80.00)
Educational level			25.62	0.00			4.31	0.03
High school or below(140)	60(42.90)	80(57.10)			28(20.00)	112(80.00)
College degree and above (107)	14(13.10)	93(86.90)			11(10.30)	96(89.70)
Annual family income			23.26	0.00			2.16	0.10
<40,000 yuan(119)	53(44.50)	66(55.50)			23(19.30)	96(80.70)
≥40,000 yuan(128)	21(16.4)	107(83.60)			16(12.50)	112(87.50)
Family support			9.16	0.00			0.39	0.33
Supported(199)	51(25.60)	148(74.40)			30(15.10)	169(84.90)
Partially supported(48)	23(47.90)	25(52.10)			9(18.80)	39(81.30)
Marital relationship			4.77	0.02			4.36	0.03
Harmony(168)	43(25.60)	125(74.40)			21(12.40)	147(87.60)
Not bad(79)	31(39.20)	48(60.80)			18(22.80)	61(77.20)

Table 2

Logistic Regression Analysis of Influencing Factors of Anxiety and Depression in Women with IVF-ET

Variables	Regression Coefficient	Standard Error	Standard Regression Coefficient	P	OR/Exp(B)	95% CI
Anxiety
Constant	−24.173	3.859	39.234	0.000
Age	0.733	0.115	40.631	0.000	2.081	1.661~2.607
Educational level	−1.621	0.461	12.348	0.000	0.198	0.080~0.488
Annual family income	−1.485	0.453	10.760	0.001	0.227	0.093~0.550
Depression
Constant	−12.361	2.713	20.759	0.000	0.000
Age	0.231	0.079	8.453	0.004	1.260	1.078~1.472
Infertility period	2.046	0.483	17.961	0.000	7.734	3.003~19.918

The Influencing Factors of Anxiety and Depression in Men Undergoing IVF-ET

In the 247 men undergoing IVF-ET, 20.65% (51/247) had anxiety, with 17.81% (44/247) having mild anxiety, and 2.83% (7/247) having moderate anxiety. Moreover, 13.77% (34/247) had depression, and in all cases, this was mild depression. The incidence of anxiety was significantly different in terms of the different types of infertility, causes of infertility, and family support (p < 0.05), and the incidence of depression was different in terms of different types of infertility, causes of infertility, and annual family income (p < 0.05) (Table 3).

Table 3

Univariate Analysis of Anxiety and Depression-Related Factors in Men with IVF-ET

Related Factors	Anxiety (n,%)		Χ2	P	Depression (n,%)		Χ2	P
	Yes	No			Yes	No
Age (y)			0.51	0.30			0.31	0.36
<30(83)	15(18.10)	68(81.90)			10(12.00)	73(88.00)
≥30(164)	36(22.00)	128(78.00)			24(14.60)	140(85.40)
Infertility period(y)			3.03	0.06			2.21	0.10
<5(138)	23(16.70)	115(83.30)			15(10.90)	123(89.10)
≥5(109)	28(25.70)	81(74.30)			19(17.40)	90(82.60)
Infertility type			30.46	0.00			18.69	0.00
Primary infertility(69)	30(43.50)	39(56.50)			20(29.00)	49(71.00)
Secondary infertility(178)	21(11.80)	157(88.20)			14(7.90)	164(92.10)
Cause of infertility			28.57	0.00			20.79	0.00
Female(118)	17(14.40)	101(85.60)			10(8.50)	108(91.50)
Male (47)	23(48.90)	24(51.20)			16(34.00)	31(66.00)
Both sides(62)	9(14.50)	53(85.50)			5(8.10)	57(91.90)
Unknown reason(20)	2(10.00)	18(90.00)			3(15.00)	17(85.00)
Educational level			0.06	0.47			0.11	0.45
High school or below(161)	34(21.10)	127(78.90)			23(14.30)	138(85.70)
College degree and above(86)	17(19.80)	69(80.20)			11(12.80)	75(87.20)
Annual family income			1.16	0.18			4.31	0.03
<40,000 yuan(119)	28(23.50)	91(76.50)			22(18.50)	97(81.50)
≥40,000 yuan(128)	23(18.00)	105(82.00)			12(9.40)	116(90.60)
Family support			4.42	0.04			0.42	0.33
Supported(199)	14(7.00)	185(93.00)			26(13.10)	173(86.90)
Partially supported(48)	8(16.70)	40(83.30)			8(16.70)	40(83.30)
Marital relationship			0.47	0.30			0.71	0.23
Harmony(168)	31(18.50)	137(81.50)			21(12.50)	147(87.50)
Not bad(79)	20(21.70)	59(78.30)			13(16.50)	66(83.50)

The Differences in the Incidence and Scores of Anxiety and Depression in Diverse Stages of IVF-ET Between Women and Men

The incidence of anxiety was 29.96%, 44.94%, and 17.81% in the T1, T2, and T3 stages, respectively. The anxiety scores were 46.14 ± 8.37, 50.83 ± 8.50, and 44.09 ± 8.17, respectively. The incidence and scores of anxiety in these three stages were significantly higher in women than in men (p < 0.05). The incidence of depression in women receiving IVF-ET was 15.79%, 9.31%, and 6.88% in the three stages, respectively. There was no significant difference in the incidence and scores of depression between women and men (Figures 2 and 3). The anxiety score in the T2 stage was the highest in women, and it was significantly higher than that in the T1 and T3 stages. The depression score of women in the T1 stage was also the highest, and it was significantly higher than in the T2 and T3 stages. In men, the incidence of anxiety in the T1, T2, and T3 stages was 20.65%, 8.50%, and 6.07%, respectively, while the incidence of depression was 13.77%, 8.91%, and 8.91%, respectively. The incidence of anxiety and depression was not significantly different in diverse stages. There was no significant difference in male anxiety and depression scores in the different stages (Figure 4).

Figure 2

Comparison of the incidence of anxiety and depression in different stages of IVF-ET between women and men. *p < 0.05 vs men.

Figure 3

Comparison of the score of anxiety and depression in different stages of IVF-ET between women and men. *p < 0.05 vs men.

Figure 4

Changes in anxiety and depression scores in different stages of IVF-ET in women/men. The anxiety score in the T2 stage was the highest in women, and it was significantly higher than that in stages T1 and T3. The depression score of women in the T1 stage was the highest, and it was significantly higher than in stages T2 and T3. *p < 0.05 vs T1; #p < 0.05 vs T2.

The Association Between Anxiety and Depression in Women/Men and IVF-ET Outcomes

In this study, 117 cases of pregnancy and 130 cases of non-pregnancy were examined. We found that there was no significant difference in anxiety and depression scores between the pregnancy and non-pregnancy groups (Figure 5).

Figure 5

Comparison of anxiety and depression scores in different IVF-ET outcomes.

Discussion

In this study, we found that the incidence of anxiety and depression in infertile couples undergoing IVF-ET is related to many factors, such as types of infertility, and family income. We also found that the incidence and scores of anxiety and depression are different in diverse stages of IVF-ET, especially in women. The anxiety score in stage T2 was the highest in women, and the depression score of women in stage T1 was the highest.

Infertile couples in China frequently experience pressure from traditional ideals, families, individual aspects, and other aspects, which leads to anxiety, depression, and other psychological disorders.6,7 This study found that the incidence of anxiety and depression on the first day of entering the IVF-ET cycle in infertile women was 29.96% and 15.79%, respectively, which was similar to the findings reported by Huang et al.8,9 Logistic regression analysis indicated that the incidence of anxiety in women was related to age, education level, and annual family income, and the incidence of depression was related to age and infertility period, which was consistent with the report of Xu and other reports.10–13 The incidence of anxiety and depression in men undergoing IVF-ET was 20.65% and 13.77%, respectively, and it was 43.50% and 29.00%, respectively, in men with primary infertility, which was significantly higher than in men with secondary infertility. However, the findings in men with male infertile factors were 48.90% and 34.00%, respectively, which were significantly higher than those in men with other infertile factors. The occurrence of anxiety and depression in men with primary infertility or male infertile factors was higher, which may be linked to the increase in male frustration, particularly the influence of Chinese traditional ideals of success, which increases the psychological burden.

It is generally considered that women are more vulnerable to infertility than men.12,14,15 Therefore, women will experience higher levels of anxiety and panic when the cause of infertility is only female factors, which may be related to feelings of guilt.16 In this study, the incidence and score of anxiety in the three stages were significantly higher in women than in men, while the incidence and score of depression were similar, showing that the anxiety of women is generally higher than that of men during the whole IVF-ET course. Consequently, particular attention should be paid to psychological counseling for infertile women.17

Infertile couples experience different problems or confusion in the diverse stages of IVF-ET.18,19 Before entering the cycle, they are under pressure due to the history of infertility. Although IVF-ET will bring hope to them, the success rate is not 100%, which means the treatment effect is uncertain, and therefore there is a psychological burden.20,21 On the day hCG is administered, there are higher expectations of success, abdominal distension due to ovarian growth (particularly in those at high risk of OHSS), and the fear of egg retrieval surgery (particularly during the operation without general anesthesia that will increase the patients’ fear of pain and cause nervousness). After embryo-transfer, the patients are concerned about the time, money, whether the psychological efforts will have good results, and what to do if they fail.20 This study compared the changes in anxiety and depression in diverse stages in women/men undergoing IVF-ET. The results showed that the anxiety score of women was the highest on the day of hCG administration and the depression score was the highest on the day of entering the cycle, which was significantly higher than that in the other two stages. There was no significant difference in the anxiety and depression scores in men between the different periods. Awtani et al found that women receiving IVF-ET were most anxious after embryo-transfer,20 whereas in this study, women had the highest anxiety score on the day of hCG administration, which may be related to the lack of general anesthesia that increases the patients’ fear of pain during surgery. Furthermore, the correlation analysis was feasible in avoiding this effect. The depression score reduced after starting the IVF-ET course, showing that active treatment could improve the depressive state of infertile women.

The influence of the infertile couples’ psychological status on the IVF-ET outcome is a concern. This study found that there were no significant differences in anxiety and depression scores between the different IVF-ET outcomes, as reported by Maroufizadeh et al.22,23 These results indicate that anxiety and depression in infertile couples may not affect the IVF-ET outcome. However, in infertile women that experience failure in IVF-ET treatment, depression and anxiety are higher and lead to low quality of life.6,24 Therefore, psychosocial intervention and support should be provided effectively to reduce the psychological distress in these women.

Limitations

This study has some limitations, however, for example, the sample size is not large enough. Larger studies, preferably in the form of randomized controlled trials, may be conducted in future research.

Conclusions

The incidence of anxiety and depression in infertile couples undergoing IVF-ET is higher, particularly in women, and it is related to many factors. More attention should be paid to the psychological state of patients, and appropriate intervention should be provided. According to this study, women are more prone to anxiety and depression throughout IVF-ET, and therefore more attention is needed. However, our study shows that anxiety and depression are not significantly correlated with the outcome of IVF-ET.