Literature DB >> 33656678

Detecting Blue Light-Dependent Protein-Protein Interactions by LexA-Based Yeast Two-Hybrid Assay.

Xiaolong Hao1, Ling Li2.   

Abstract

The LexA-based yeast two-hybrid system is one of the most powerful techniques used to detect blue light-dependent protein-protein interactions. In Arabidopsis, many protein-protein interactions in blue light signaling pathway were identified using this system. Here we present an easy and efficient method of the LexA-based yeast two-hybrid assay for testing protein-protein interactions in a blue light-dependent manner.

Entities:  

Keywords:  Blue light signaling pathway; Blue light-dependent; LexA; Protein–protein interaction; Yeast two-hybrid assay

Year:  2021        PMID: 33656678     DOI: 10.1007/978-1-0716-1370-2_15

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN: 1064-3745


  3 in total

1.  Yeast two-hybrid vectors and strains.

Authors:  P James
Journal:  Methods Mol Biol       Date:  2001

Review 2.  Techniques for the Analysis of Protein-Protein Interactions in Vivo.

Authors:  Shuping Xing; Niklas Wallmeroth; Kenneth W Berendzen; Christopher Grefen
Journal:  Plant Physiol       Date:  2016-04-25       Impact factor: 8.340

3.  Expression of an N-terminal fragment of COP1 confers a dominant-negative effect on light-regulated seedling development in Arabidopsis.

Authors:  T W McNellis; K U Torii; X W Deng
Journal:  Plant Cell       Date:  1996-09       Impact factor: 11.277

  3 in total

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