Warning: Undefined array key "mm" in /www/wwwroot/www.ai-bt.com/si.php on line 10 Deprecated: trim(): Passing null to parameter #1 ($string) of type string is deprecated in /www/wwwroot/www.ai-bt.com/si.php on line 10 Measurement of Mitotic Spindle Angle and Mitotic Cell Distance in Fixed Tissue of Drosophila Larval Brains.

Literature DB >> 33654928

Measurement of Mitotic Spindle Angle and Mitotic Cell Distance in Fixed Tissue of Drosophila Larval Brains.

Abstract

The positioning and the cleavage plane orientation of mitotic cells in pseudostratified epithelia (PSE) must be tightly regulated since failures in any of these processes might have fatal consequences during development. Here we present a simple method to determine the spindle orientation as well as the positioning of neuroepithelial mitotic cells within the Outer Proliferation Center (OPC) of Drosophila larval brains.

Entities: Chemical

Keywords: Drosophila; Interkinetic nuclear migration; Mitosis; Neuroepithelial cells; Proliferation; Pseudostratified Epithelia; Spindle orientation

Year: 2019 PMID： 33654928 PMCID： PMC7854012 DOI： 10.21769/BioProtoc.3432

Source DB: PubMed Journal: Bio Protoc ISSN： 2331-8325

Keyword Cloud
References

11 in total

1. Analysis and modeling of mitotic spindle orientations in three dimensions.

Authors: Christoph Jüschke; Yunli Xie; Maria Pia Postiglione; Juergen A Knoblich
Journal: Proc Natl Acad Sci U S A Date: 2013-12-31 Impact factor: 11.205

2. Interkinetic nuclear migration is a broadly conserved feature of cell division in pseudostratified epithelia.

Authors: Emily J Meyer; Aissam Ikmi; Matthew C Gibson
Journal: Curr Biol Date: 2011-03-03 Impact factor: 10.834

Review 3. Pseudostratified epithelia - cell biology, diversity and roles in organ formation at a glance.

Authors: Caren Norden
Journal: J Cell Sci Date: 2017-04-28 Impact factor: 5.285

Review 4. Heterogeneity, Cell Biology and Tissue Mechanics of Pseudostratified Epithelia: Coordination of Cell Divisions and Growth in Tightly Packed Tissues.

Authors: P J Strzyz; M Matejcic; C Norden
Journal: Int Rev Cell Mol Biol Date: 2016-04-20 Impact factor: 6.813

5. Mitotic spindle misorientation in cancer--out of alignment and into the fire.

Authors: Jillian C Pease; Jennifer S Tirnauer
Journal: J Cell Sci Date: 2011-04-01 Impact factor: 5.285

6. FAK-mediated extracellular signals are essential for interkinetic nuclear migration and planar divisions in the neuroepithelium.

Authors: Sachiko Tsuda; Tadao Kitagawa; Shigeo Takashima; Shuichi Asakawa; Nobuyoshi Shimizu; Hiroshi Mitani; Akihiro Shima; Makiko Tsutsumi; Hiroshi Hori; Kiyoshi Naruse; Yuji Ishikawa; Hiroyuki Takeda
Journal: J Cell Sci Date: 2010-01-12 Impact factor: 5.285

Measurement of Mitotic Spindle Angle and Mitotic Cell Distance in Fixed Tissue of Drosophila Larval Brains.

1. Analysis and modeling of mitotic spindle orientations in three dimensions.

2. Interkinetic nuclear migration is a broadly conserved feature of cell division in pseudostratified epithelia.

Review 3. Pseudostratified epithelia - cell biology, diversity and roles in organ formation at a glance.

Review 4. Heterogeneity, Cell Biology and Tissue Mechanics of Pseudostratified Epithelia: Coordination of Cell Divisions and Growth in Tightly Packed Tissues.

5. Mitotic spindle misorientation in cancer--out of alignment and into the fire.

6. FAK-mediated extracellular signals are essential for interkinetic nuclear migration and planar divisions in the neuroepithelium.

Review 7. Mitotic spindle (DIS)orientation and DISease: cause or consequence?

8. Cell shape impacts on the positioning of the mitotic spindle with respect to the substratum.

9. Eph signaling controls mitotic spindle orientation and cell proliferation in neuroepithelial cells.

10. Live imaging of whole mouse embryos during gastrulation: migration analyses of epiblast and mesodermal cells.