Literature DB >> 33654915

Adoptive Transfer of Basophils Enriched from Mouse Spleen.

Adrian M Piliponsky1,2,3, Stephen J Galli4,5,6.   

Abstract

CD49b is a member of the integrin family, expressed on basophils, natural killer (NK) cells and a subset CD4+ T cells in the spleen. This protocol describes the adoptive transfer of basophil-enriched CD49b+ cells obtained from mouse spleens by magnetic enrichment. This protocol can be used to assess the contribution of basophils or basophil-derived mediators to a certain immune response.
Copyright © 2019 The Authors; exclusive licensee Bio-protocol LLC.

Entities:  

Keywords:  Adoptive transfer; Basophils; Magnetic enrichment; Mouse; Spleen

Year:  2019        PMID： 33654915      PMCID： PMC7853924          DOI： 10.21769/BioProtoc.3416

Source DB:  PubMed          Journal:  Bio Protoc        ISSN： 2331-8325


  2 in total

1.  Selective ablation of basophils in mice reveals their nonredundant role in acquired immunity against ticks.

Authors:  Takeshi Wada; Kenji Ishiwata; Haruhiko Koseki; Tomoyuki Ishikura; Tsukasa Ugajin; Naotsugu Ohnuma; Kazushige Obata; Ryosuke Ishikawa; Soichiro Yoshikawa; Kaori Mukai; Yohei Kawano; Yoshiyuki Minegishi; Hiroo Yokozeki; Naohiro Watanabe; Hajime Karasuyama
Journal:  J Clin Invest       Date:  2010-07-26       Impact factor: 14.808

2.  Basophil-derived tumor necrosis factor can enhance survival in a sepsis model in mice.

Authors:  Adrian M Piliponsky; Nicholas J Shubin; Asha K Lahiri; Phuong Truong; Morgan Clauson; Kerri Niino; Avery L Tsuha; Sergei A Nedospasov; Hajime Karasuyama; Laurent L Reber; Mindy Tsai; Kaori Mukai; Stephen J Galli
Journal:  Nat Immunol       Date:  2019-01-21       Impact factor: 25.606
  2 in total

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