Gas chromatography/mass spectrometry and gas chromatography/tandem mass spectrometry of methyl ester/methoxime/trimethylsilyl ether derivatives of prostaglandins.

Electron impact mass spectra of methyl ester/trimethylsilyl ether derivatives of prostaglandin F2 alpha and methyl ester/methoxime/trimethylsilyl ether derivatives of prostaglandin E2, prostaglandin D2, 6-oxo-prostaglandin F1 alpha and 2,3-dinor-6-oxo-prostaglandin F1 alpha are presented. Most of the prostaglandins studied have additionally been 2H-labelled at different sites in order to assign the corresponding fragment ions. Collisionally activated decomposition mass spectra of the most intense parent ions in the high-mass region were taken. High-intensity, prostaglandin-characteristic daughter fragments will allow a reliable quantification of prostaglandins in biological fluids and a reduction of sample clean-up.