Literature DB >> 33654796

Production and Purification of Dengue Virus-like Particles from COS-1 Cells.

Jedhan Ucat Galula1, Gwong-Jen J Chang2, Day-Yu Chao1.   

Abstract

Non-infectious virus-like particles (VLPs) containing dengue virus (DENV) pre-membrane (prM) and envelope (E) proteins have been demonstrated to be highly immunogenic and can be used as a potential vaccine candidate as well as a tool for serodiagnostic assays. Successful application of VLPs requires abundant, and high-purity production methods. Here, we describe a robust protocol for producing DENV VLPs from transiently-transformed or stable COS-1 cells and further provide an easily adaptable antigen purification method by sucrose gradient centrifugation. ©Copyright Galula et al.

Entities:  

Keywords:  COS-1 cell; Dengue virus (DENV); Sucrose gradient centrifugation; VLP purification; Virus-like particle (VLP)

Year:  2019        PMID： 33654796      PMCID： PMC7854098          DOI： 10.21769/BioProtoc.3280

Source DB:  PubMed          Journal:  Bio Protoc        ISSN： 2331-8325


  13 in total

1.  Comparative analysis of immunoglobulin M (IgM) capture enzyme-linked immunosorbent assay using virus-like particles or virus-infected mouse brain antigens to detect IgM antibody in sera from patients with evident flaviviral infections.

Authors:  Derek A Holmes; David E Purdy; Day-Yu Chao; Amanda J Noga; Gwong-Jen J Chang
Journal:  J Clin Microbiol       Date:  2005-07       Impact factor: 5.948

2.  Enhancing biosynthesis and secretion of premembrane and envelope proteins by the chimeric plasmid of dengue virus type 2 and Japanese encephalitis virus.

Authors:  Gwong-Jen J Chang; Ann R Hunt; Derek A Holmes; Tracy Springfield; Tzong-Shi Chiueh; John T Roehrig; Duane J Gubler
Journal:  Virology       Date:  2003-02-01       Impact factor: 3.616

3.  A recombinant particulate antigen of Japanese encephalitis virus produced in stably-transformed cells is an effective noninfectious antigen and subunit immunogen.

Authors:  A R Hunt; C B Cropp; G J Chang
Journal:  J Virol Methods       Date:  2001-09       Impact factor: 2.014

4.  Comprehensive Evaluation of Differential Serodiagnosis between Zika and Dengue Viral Infections.

Authors:  Day-Yu Chao; Matthew T Whitney; Brent S Davis; Freddy A Medina; Jorge L Munoz; Gwong-Jen J Chang
Journal:  J Clin Microbiol       Date:  2019-02-27       Impact factor: 5.948

5.  A single intramuscular injection of recombinant plasmid DNA induces protective immunity and prevents Japanese encephalitis in mice.

Authors:  G J Chang; A R Hunt; B Davis
Journal:  J Virol       Date:  2000-05       Impact factor: 5.103

6.  West Nile virus recombinant DNA vaccine protects mouse and horse from virus challenge and expresses in vitro a noninfectious recombinant antigen that can be used in enzyme-linked immunosorbent assays.

Authors:  B S Davis; G J Chang; B Cropp; J T Roehrig; D A Martin; C J Mitchell; R Bowen; M L Bunning
Journal:  J Virol       Date:  2001-05       Impact factor: 5.103

7.  Virus-like particle secretion and genotype-dependent immunogenicity of dengue virus serotype 2 DNA vaccine.

Authors:  Jedhan U Galula; Wen-Fan Shen; Shih-Te Chuang; Gwong-Jen J Chang; Day-Yu Chao
Journal:  J Virol       Date:  2014-07-09       Impact factor: 5.103

8.  Tetravalent recombinant dengue virus-like particles as potential vaccine candidates: immunological properties.

Authors:  Yan Liu; Junmei Zhou; Zhizhun Yu; Danyun Fang; Chunyun Fu; Xun Zhu; Zhenjian He; Huijun Yan; Lifang Jiang
Journal:  BMC Microbiol       Date:  2014-12-18       Impact factor: 3.605

9.  Humoral immune responses of dengue fever patients using epitope-specific serotype-2 virus-like particle antigens.

Authors:  Wayne D Crill; Holly R Hughes; Mark J Delorey; Gwong-Jen J Chang
Journal:  PLoS One       Date:  2009-04-01       Impact factor: 3.240

10.  Epitope resurfacing on dengue virus-like particle vaccine preparation to induce broad neutralizing antibody.

Authors:  Wen-Fan Shen; Jedhan Ucat Galula; Jyung-Hurng Liu; Mei-Ying Liao; Day-Yu Chao; Cheng-Hao Huang; Yu-Chun Wang; Han-Chung Wu; Jian-Jong Liang; Yi-Ling Lin; Matthew T Whitney; Gwong-Jen J Chang; Sheng-Ren Chen; Shang-Rung Wu
Journal:  Elife       Date:  2018-10-18       Impact factor: 8.140
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