Literature DB >> 33654740

Mouse Embryonic Tooth Germ Dissection and Ex vivo Culture Protocol.

Xue Han1, Keigo Yoshizaki1, Tian Tian1, Kanako Miyazaki1, Ichiro Takahashi1, Satoshi Fukumoto2,3.   

Abstract

A tooth germ ex vivo organ culture allows visualization of its development in different stages, thus enabling investigation of the molecular mechanisms of regulatory factors. Tooth germs can be rapidly dissected from E13 mouse embryos and placed on cell culture inserts for observation of subsequent tooth germ development in a three-dimensional situation in real time. This method is also suitable for other organs that develop by epithelial-mesenchymal interactions, including salivary gland, hair, lung, and kidney. In addition, siRNAs or growth factors can be easily added to ex vivo tooth germ cultures to investigate the detailed molecular function of specific genes. The present protocol provides an efficient and practical method for isolation and ex vivo culture of embryonic tooth germs.
Copyright © 2020 The Authors; exclusive licensee Bio-protocol LLC.

Entities:  

Keywords:  Embryonic tooth germ isolation; Epithelial-mesenchymal interaction; Ex vivo culture ; Organ culture; Organogenesis; Tooth development

Year:  2020        PMID： 33654740      PMCID： PMC7842286          DOI： 10.21769/BioProtoc.3515

Source DB:  PubMed          Journal:  Bio Protoc        ISSN： 2331-8325


  10 in total

1.  Laminin alpha5 is required for dental epithelium growth and polarity and the development of tooth bud and shape.

Authors:  Satoshi Fukumoto; Jeffrey H Miner; Hiroko Ida; Emiko Fukumoto; Kenji Yuasa; Hiroshi Miyazaki; Matthew P Hoffman; Yoshihiko Yamada
Journal:  J Biol Chem       Date:  2005-12-19       Impact factor: 5.157

2.  Ultrastructural analysis of enamel formation during in vitro development using chemically-defined medium.

Authors:  P Bringas; M Nakamura; E Nakamura; J Evans; H C Slavkin
Journal:  Scanning Microsc       Date:  1987-09

3.  Chemically-defined organ culture of embryonic mouse tooth organs: morphogenesis, dentinogenesis and amelogenesis.

Authors:  M Yamada; P Bringas; M Grodin; M MacDougall; E Cummings; J Grimmett; B Weliky; H C Slavkin
Journal:  J Biol Buccale       Date:  1980-06

4.  The ultrastructure of the rat incisor odontoblast in organ culture.

Authors:  B R Heywood; J Appleton
Journal:  Arch Oral Biol       Date:  1984       Impact factor: 2.633

5.  Pancreas development ex vivo: culturing embryonic pancreas explants on permeable culture inserts, with fibronectin-coated glass microwells, or embedded in three-dimensional Matrigel™.

Authors:  Hung Ping Shih; Maike Sander
Journal:  Methods Mol Biol       Date:  2014

6.  The transcription factor NKX2-3 mediates p21 expression and ectodysplasin-A signaling in the enamel knot for cusp formation in tooth development.

Authors:  Xue Han; Keigo Yoshizaki; Kanako Miyazaki; Chieko Arai; Keita Funada; Tomomi Yuta; Tian Tian; Yuta Chiba; Kan Saito; Tsutomu Iwamoto; Aya Yamada; Ichiro Takahashi; Satoshi Fukumoto
Journal:  J Biol Chem       Date:  2018-08-08       Impact factor: 5.157

7.  Connexin 43 Is Necessary for Salivary Gland Branching Morphogenesis and FGF10-induced ERK1/2 Phosphorylation.

Authors:  Aya Yamada; Masaharu Futagi; Emiko Fukumoto; Kan Saito; Keigo Yoshizaki; Masaki Ishikawa; Makiko Arakaki; Ryoko Hino; Yu Sugawara; Momoko Ishikawa; Masahiro Naruse; Kanako Miyazaki; Takashi Nakamura; Satoshi Fukumoto
Journal:  J Biol Chem       Date:  2015-11-12       Impact factor: 5.157

8.  Platelet-derived growth factor receptor regulates salivary gland morphogenesis via fibroblast growth factor expression.

Authors:  Shinya Yamamoto; Emiko Fukumoto; Keigo Yoshizaki; Tsutomu Iwamoto; Aya Yamada; Kojiro Tanaka; Hiroharu Suzuki; Shizuko Aizawa; Makiko Arakaki; Kenji Yuasa; Kyoko Oka; Yang Chai; Kazuaki Nonaka; Satoshi Fukumoto
Journal:  J Biol Chem       Date:  2008-06-17       Impact factor: 5.157

9.  Hepatocyte growth factor is involved in the morphogenesis of tooth germ in murine molars.

Authors:  M J Tabata; K Kim; J G Liu; K Yamashita; T Matsumura; J Kato; M Iwamoto; S Wakisaka; K Matsumoto; T Nakamura; M Kumegawa; K Kurisu
Journal:  Development       Date:  1996-04       Impact factor: 6.868

10.  The Three-Dimensional Culture System with Matrigel and Neurotrophic Factors Preserves the Structure and Function of Spiral Ganglion Neuron In Vitro.

Authors:  Gaoying Sun; Wenwen Liu; Zhaomin Fan; Daogong Zhang; Yuechen Han; Lei Xu; Jieyu Qi; Shasha Zhang; Bradley T Gao; Xiaohui Bai; Jianfeng Li; Renjie Chai; Haibo Wang
Journal:  Neural Plast       Date:  2016-01-06       Impact factor: 3.599
  10 in total
  1 in total

1.  An ex vivo organ culture screening model revealed that low temperature conditions prevent side effects of anticancer drugs.

Authors:  Tian Tian; Kanako Miyazaki; Yuta Chiba; Keita Funada; Tomomi Yuta; Kanji Mizuta; Yao Fu; Jumpei Kawahara; Xue Han; Yuna Ando; Ami Funada; Aya Yamada; Tsutomu Iwamoto; Seiji Nakamura; Ichiro Takahashi; Satoshi Fukumoto; Keigo Yoshizaki
Journal:  Sci Rep       Date:  2022-02-23       Impact factor: 4.379
  1 in total

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