Literature DB >> 33654722

Sulfatase Assay to Determine Influence of Plants on Microbial Activity in Soil.

Anna Koprivova1, Achim Schmalenberger2, Stanislav Kopriva1.   

Abstract

Sulfatase activity is often used as a measure of the activity of soil microorganisms. It is thus a suitable tool to investigate the response of microbes to plants. Here we present a method to determine the influence of various Arabidopsis genotypes on the function of soil microbiota using the sulfatase as a quantitative measure. We grew the plants in soil/sand mix under control conditions and measured the sulfatase activity in soil using a spectrophotometric determination of the product. This protocol can be used to test the contribution of individual genes to control of microbiome assembly through analysis of mutants as well as the influence of environment on plant-microbe interactions.
Copyright © 2020 The Authors; exclusive licensee Bio-protocol LLC.

Entities:  

Keywords:  Arabidopsis; Bacteria; Microbiome; Organic sulfur; Plant-microbe interactions; Soil; Sulfatase

Year:  2020        PMID： 33654722      PMCID： PMC7842368          DOI： 10.21769/BioProtoc.3490

Source DB:  PubMed          Journal:  Bio Protoc        ISSN： 2331-8325


  12 in total

1.  Functional overlap of the Arabidopsis leaf and root microbiota.

Authors:  Yang Bai; Daniel B Müller; Girish Srinivas; Ruben Garrido-Oter; Eva Potthoff; Matthias Rott; Nina Dombrowski; Philipp C Münch; Stijn Spaepen; Mitja Remus-Emsermann; Bruno Hüttel; Alice C McHardy; Julia A Vorholt; Paul Schulze-Lefert
Journal:  Nature       Date:  2015-12-02       Impact factor: 49.962

Review 2.  Structure and functions of the bacterial microbiota of plants.

Authors:  Davide Bulgarelli; Klaus Schlaeppi; Stijn Spaepen; Emiel Ver Loren van Themaat; Paul Schulze-Lefert
Journal:  Annu Rev Plant Biol       Date:  2013-01-30       Impact factor: 26.379

Review 3.  Sulfation pathways from red to green.

Authors:  Süleyman Günal; Rebecca Hardman; Stanislav Kopriva; Jonathan Wolf Mueller
Journal:  J Biol Chem       Date:  2019-07-02       Impact factor: 5.157

Review 4.  The role of soil microbes in plant sulphur nutrition.

Authors:  Michael A Kertesz; Pascal Mirleau
Journal:  J Exp Bot       Date:  2004-06-04       Impact factor: 6.992

5.  Revealing structure and assembly cues for Arabidopsis root-inhabiting bacterial microbiota.

Authors:  Davide Bulgarelli; Matthias Rott; Klaus Schlaeppi; Emiel Ver Loren van Themaat; Nahal Ahmadinejad; Federica Assenza; Philipp Rauf; Bruno Huettel; Richard Reinhardt; Elmon Schmelzer; Joerg Peplies; Frank Oliver Gloeckner; Rudolf Amann; Thilo Eickhorst; Paul Schulze-Lefert
Journal:  Nature       Date:  2012-08-02       Impact factor: 49.962

Review 6.  The role of bacteria and mycorrhiza in plant sulfur supply.

Authors:  Jacinta Gahan; Achim Schmalenberger
Journal:  Front Plant Sci       Date:  2014-12-16       Impact factor: 5.753

7.  MYB72-dependent coumarin exudation shapes root microbiome assembly to promote plant health.

Authors:  Ioannis A Stringlis; Ke Yu; Kirstin Feussner; Ronnie de Jonge; Sietske Van Bentum; Marcel C Van Verk; Roeland L Berendsen; Peter A H M Bakker; Ivo Feussner; Corné M J Pieterse
Journal:  Proc Natl Acad Sci U S A       Date:  2018-04-23       Impact factor: 11.205

8.  Root-specific camalexin biosynthesis controls the plant growth-promoting effects of multiple bacterial strains.

Authors:  Anna Koprivova; Stefan Schuck; Richard P Jacoby; Irene Klinkhammer; Bastian Welter; Lisa Leson; Anna Martyn; Julia Nauen; Niklas Grabenhorst; Jan F Mandelkow; Alga Zuccaro; Jürgen Zeier; Stanislav Kopriva
Journal:  Proc Natl Acad Sci U S A       Date:  2019-07-16       Impact factor: 11.205

9.  Long-term monitoring of soil microbiological activities in two forest sites in South tyrol in the italian alps.

Authors:  Rosa Margesin; Stefano Minerbi; Franz Schinner
Journal:  Microbes Environ       Date:  2014-07-10       Impact factor: 2.912

10.  Biochemical and microbiological activity of soil contaminated with o-cresol and biostimulated with Perna canaliculus mussel meal.

Authors:  Magdalena Zaborowska; Jan Kucharski; Jadwiga Wyszkowska
Journal:  Environ Monit Assess       Date:  2018-09-21       Impact factor: 2.513
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