Literature DB >> 33650946

Elevation of Lactococcus lactis subsp. cremoris to the species level as Lactococcus cremoris sp. nov. and transfer of Lactococcus lactis subsp. tructae to Lactococcus cremoris as Lactococcus cremoris subsp. tructae comb. nov.

Ting Ting Li1,2, Wen Li Tian2, Chun Tao Gu2,1.   

Abstract

Currently, Lactococcus lactis contains four subspecies: L. lactis subsp. lactis, L. lactis subsp. hordniae, L. lactis subsp. cremoris and L. lactis subsp. tructae. In the study of Pérez et al., these four subspecies could be clearly divided into two groups based on recA sequence analysis: L. lactis subsp. lactis and L. lactis subsp. hordniae; L. lactis subsp. cremoris and L. lactis subsp. tructae. The two groups had a relatively low DNA-DNA hybridization value (about 60 %). In the present study, the taxonomic position of L. lactis subsp. cremoris and L. lactis subsp. tructae was re-examined based on sequence analyses of 16S rRNA, rpoB, recA and pheS genes, average nucleotide identity (ANI) values and digital DNA-DNA hybridization (dDDH) values. The result of 16S rRNA gene sequence analysis indicated that L. lactis subsp. cremoris NCDO 607T and L. lactis subsp. tructae L105T were phylogenetically related to the type strains of L. lactis subsp. hordniae, L. lactis subsp. lactis, Lactococcus taiwanensis, Lactococcus kimchii, Lactococcus allomyrinae, Lactococcus protaetiae, Lactococcus hircilactis, Lactococcus fujiensis and Lactococcus nasutitermitis. The 16S rRNA gene, rpoB, recA, pheS and concatenated rpoB, recA and pheS sequence similarities, ANI values, and dDDH values between the type strains of L. lactis subsp. cremoris, L. lactis subsp. tructae and phylogenetically related species were 93.5-99.4 %, 83.3-97.6 %, 80.6-92.4 %, 79.7-92.7 %, 83.5-94.3 %, 72.4-86.9 % and 21.4-32.5 %, respectively. Lower than 95-96 % ANI values and lower than 70 % dDDH values confirmed that the type strains of L. lactis subsp. cremoris and L. lactis subsp. tructae represent a novel species in the genus Lactococcus. Because L. lactis subsp. cremoris was proposed and validated before L. lactis subsp. tructae, L. lactis subsp. cremoris is elevated to the species level as Lactococcus cremoris sp. nov. and L. lactis subsp. tructae is transferred to L. cremoris as L. cremoris subsp. tructae comb. nov. The type strain of L. cremoris sp. nov. is NCDO 607T (=ATCC 19257T=DSM 20069T=JCM 16167T=LMG 6897T=NBRC 100676T). The type strain of L. cremoris subsp. tructae comb. nov. is L105T (=NBRC 110453T=DSM 21502T=JCM 31125T=LMG 24662T).

Entities:  

Keywords:  Lactococcus; genome; reclassification

Year:  2021        PMID： 33650946     DOI： 10.1099/ijsem.0.004727

Source DB:  PubMed          Journal:  Int J Syst Evol Microbiol        ISSN： 1466-5026            Impact factor:   2.747


  9 in total

1.  Adaptive Laboratory Evolution as a Means To Generate Lactococcus lactis Strains with Improved Thermotolerance and Ability To Autolyze.

Authors:  Robin Dorau; Jun Chen; Jianming Liu; Peter Ruhdal Jensen; Christian Solem
Journal:  Appl Environ Microbiol       Date:  2021-08-18       Impact factor: 4.792

2.  Characterization of two new strains of Lactococcus lactis for their probiotic efficacy over commercial synbiotics consortia.

Authors:  Biplab Bandyopadhyay; Satinath Das; Prashanta Kumar Mitra; Ashutosh Kundu; Vivekananda Mandal; Rajsekhar Adhikary; Vivekananda Mandal; Narayan Chandra Mandal
Journal:  Braz J Microbiol       Date:  2022-02-09       Impact factor: 2.214

3.  Stationary Lactococcus cremoris: Energetic State, Protein Synthesis Without Nitrogen and Their Effect on Survival.

Authors:  Sieze Douwenga; Rinke J van Tatenhove-Pel; Emile Zwering; Herwig Bachmann
Journal:  Front Microbiol       Date:  2021-12-17       Impact factor: 5.640

4.  Characterization of exopolysaccharide-producing lactic acid bacteria from Taiwanese ropy fermented milk and their application in low-fat fermented milk.

Authors:  Ker-Sin Ng; Yu-Chun Chang; Yen-Po Chen; Ya-Hsuan Lo; Sheng-Yao Wang; Ming-Ju Chen
Journal:  Anim Biosci       Date:  2021-08-25

5.  Cell Surface Polysaccharides Represent a Common Strategy for Adsorption among Phages Infecting Lactic Acid Bacteria: Lessons from Dairy Lactococci and Streptococci.

Authors:  Jennifer Mahony
Journal:  mSystems       Date:  2021-08-17       Impact factor: 6.496

6.  Physiological Roles of Short-Chain and Long-Chain Menaquinones (Vitamin K2) in Lactococcus cremoris.

Authors:  Yue Liu; Nikolaos Charamis; Sjef Boeren; Joost Blok; Alisha Geraldine Lewis; Eddy J Smid; Tjakko Abee
Journal:  Front Microbiol       Date:  2022-03-15       Impact factor: 5.640

7.  Prolonged Lifespan, Improved Perception, and Enhanced Host Defense of Caenorhabditis elegans by Lactococcus cremoris subsp. cremoris.

Authors:  Tomomi Komura; Asami Takemoto; Hideki Kosaka; Toshio Suzuki; Yoshikazu Nishikawa
Journal:  Microbiol Spectr       Date:  2022-05-16

8.  Isolation and Identification of Lactic Acid Bacteria from Natural Whey Cultures of Buffalo and Cow Milk.

Authors:  Rosangela Marasco; Mariagiovanna Gazzillo; Nicoletta Campolattano; Margherita Sacco; Lidia Muscariello
Journal:  Foods       Date:  2022-01-16

9.  Genomic Features and Construction of Streamlined Genome Chassis of Nisin Z Producer Lactococcus lactis N8.

Authors:  Wanjin Qiao; Fulu Liu; Xing Wan; Yu Qiao; Ran Li; Zhenzhou Wu; Per Erik Joakim Saris; Haijin Xu; Mingqiang Qiao
Journal:  Microorganisms       Date:  2021-12-27
  9 in total

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