Jyosna Khanam1, Delowar Hossain2, Bayejid Hosen2,3, Mesbah Uddin4, Asadul Kabir5, Mohammad Anwarul Bari5. 1. Institute of Nutrition and Food Science, University of Dhaka, Bangladesh. 2. Department of Biochemistry and Molecular Biology, University of Dhaka, Dhaka, Bangladesh. 3. National Forensic DNA Profiling Laboratory, Dhaka Medical College, Dhaka, Bangladesh. 4. Clinical Pathology Laboratory, Dhaka Medical College Hospital, Dhaka, Bangladesh. 5. Department of Medicine, Sir Salimullah Medical College, Dhaka, Bangladesh.
Abstract
BACKGROUND: Myocardial infarction is one of the leading causes of morbidity and mortality worldwide. Oxidative stress plays a vital role in the pathogenesis of atherosclerosis leading to myocardial infarction and Glutathione S-transferases (GSTs) act as detoxifying enzymes to reduce oxidative stress. The aim of the present study was to investigate the associations of the GST (T1 & M1) gene polymorphism with the susceptibility of myocardial infarction in the Bangladeshi population. METHODS: A case-control study on 100 cardiac patients with MI and 150 control subjects was conducted. The genotyping of GST (T1 & M1) gene was done using conventional Polymerase Chain Reaction. RESULTS: The percentage of GSTM1 genotypes was significantly (p< 0.01) lower in patients compared to control subjects while the GSTT1 genotypes were not significantly different between the study subjects. The individual with GSTM1 null allele was at 2.5-fold increased risk {odds ratio (OR)= 2.5; 95 % confidence interval (95 % CI)= 1.4 to 4.3; p< 0.01} of experiencing MI while individual with either GSTM1 or GSTT1 genotypes was at lower risk. In the case of GST M1 and GST T1 combined genotype, patients having both null genotypes for GST M1 and GST T1 gene showed significantly (p< 0.01) higher risk of experiencing MI when compared to control subjects (OR= 3.5; 95% CI= 1.7-7.2; p< 0.001). CONCLUSION: Thus our recent study suggested that GSTM1 alone and GSTM1 and T1 in combination augments the risk of MI in Bangladeshi population.
BACKGROUND: Myocardial infarction is one of the leading causes of morbidity and mortality worldwide. Oxidative stress plays a vital role in the pathogenesis of atherosclerosis leading to myocardial infarction and Glutathione S-transferases (GSTs) act as detoxifying enzymes to reduce oxidative stress. The aim of the present study was to investigate the associations of the GST (T1 & M1) gene polymorphism with the susceptibility of myocardial infarction in the Bangladeshi population. METHODS: A case-control study on 100 cardiac patients with MI and 150 control subjects was conducted. The genotyping of GST (T1 & M1) gene was done using conventional Polymerase Chain Reaction. RESULTS: The percentage of GSTM1 genotypes was significantly (p< 0.01) lower in patients compared to control subjects while the GSTT1 genotypes were not significantly different between the study subjects. The individual with GSTM1 null allele was at 2.5-fold increased risk {odds ratio (OR)= 2.5; 95 % confidence interval (95 % CI)= 1.4 to 4.3; p< 0.01} of experiencing MI while individual with either GSTM1 or GSTT1 genotypes was at lower risk. In the case of GST M1 and GST T1 combined genotype, patients having both null genotypes for GST M1 and GST T1 gene showed significantly (p< 0.01) higher risk of experiencing MI when compared to control subjects (OR= 3.5; 95% CI= 1.7-7.2; p< 0.001). CONCLUSION: Thus our recent study suggested that GSTM1 alone and GSTM1 and T1 in combination augments the risk of MI in Bangladeshi population.
Authors: M Cicoira; L Zanolla; A Rossi; G Golia; L Franceschini; G Cabrini; A Bonizzato; M Graziani; S D Anker; A J Coats; P Zardini Journal: J Am Coll Cardiol Date: 2001-06-01 Impact factor: 24.094
Authors: Eric A Miller; James S Pankow; Robert C Millikan; Molly S Bray; Christie M Ballantyne; Douglas A Bell; Gerardo Heiss; Rongling Li Journal: Atherosclerosis Date: 2003-12 Impact factor: 5.162