Nasrin Amiri-Dashatan1, Mehdi Koushki2, Mostafa Rezaei-Tavirani1, Nayebalia Ahmadi1,3. 1. Proteomics Research Center, Faculty of Paramedical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran. 2. Department of Clinical Biochemistry, School of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran. 3. Department of Medical Lab Technology, Faculty of Paramedical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Abstract
BACKGROUND: Leishmania (L) major and L. tropica are the etiological agents of cutaneous leishmaniosis. Leishmania species cause a board spectrum of phenotypes. A small number of genes are differentially expressed between them that have likely an important role in the disease phenotype. Procyclic and metacyclic are two morphological promastigote forms of Leishmania that express different genes. The glutathione peroxidase is an important antioxidant enzyme that essential in parasite protection against oxidative stress and parasite survival. This study aimed to compare glutathione peroxidase (TDPX) gene expression in procyclic and metacyclic and also interspecies in Iranian isolates of L. major and L. tropica. METHODS: The samples were cultured in Novy-Nicolle-Mc Neal medium to obtain the promastigotes and identified using PCR-RFLP technique. They were then grown in RPMI1640 media for mass cultivation. The expression level of TDPX gene was compared by Real-time PCR. RESULTS: By comparison of expression level, up-regulation of TDPX gene was observed (5.37 and 2.29 folds) in L. major and L. tropica metacyclic compared to their procyclic, respectively. Moreover, there was no significant difference between procyclic forms of isolates, while 3.05 folds up-regulation in metacyclic was detected in L. major compared L. tropica. CONCLUSION: Our data provide a foundation for identifying infectivity and high survival related factors in the Leishmania spp. In addition, the results improve our understanding of the molecular basis of metacyclogenesis and development of new potential targets to control or treatment and also, to the identification of species-specific factors contributing to virulence and pathogenicity in the host cells.
BACKGROUND: Leishmania (L) major and L. tropica are the etiological agents of cutaneous leishmaniosis. Leishmania species cause a board spectrum of phenotypes. A small number of genes are differentially expressed between them that have likely an important role in the disease phenotype. Procyclic and metacyclic are two morphological promastigote forms of Leishmania that express different genes. The glutathione peroxidase is an important antioxidant enzyme that essential in parasite protection against oxidative stress and parasite survival. This study aimed to compare glutathione peroxidase (TDPX) gene expression in procyclic and metacyclic and also interspecies in Iranian isolates of L. major and L. tropica. METHODS: The samples were cultured in Novy-Nicolle-Mc Neal medium to obtain the promastigotes and identified using PCR-RFLP technique. They were then grown in RPMI1640 media for mass cultivation. The expression level of TDPX gene was compared by Real-time PCR. RESULTS: By comparison of expression level, up-regulation of TDPX gene was observed (5.37 and 2.29 folds) in L. major and L. tropica metacyclic compared to their procyclic, respectively. Moreover, there was no significant difference between procyclic forms of isolates, while 3.05 folds up-regulation in metacyclic was detected in L. major compared L. tropica. CONCLUSION: Our data provide a foundation for identifying infectivity and high survival related factors in the Leishmania spp. In addition, the results improve our understanding of the molecular basis of metacyclogenesis and development of new potential targets to control or treatment and also, to the identification of species-specific factors contributing to virulence and pathogenicity in the host cells.
Entities:
Keywords:
Glutathione peroxidase; L. major; L. tropica; Leishmania; Quantitative Real-time PCR
Authors: A Saxena; T Lahav; N Holland; G Aggarwal; A Anupama; Y Huang; H Volpin; P J Myler; D Zilberstein Journal: Mol Biochem Parasitol Date: 2006-12-12 Impact factor: 1.759
Authors: Christopher S Peacock; Kathy Seeger; David Harris; Lee Murphy; Jeronimo C Ruiz; Michael A Quail; Nick Peters; Ellen Adlem; Adrian Tivey; Martin Aslett; Arnaud Kerhornou; Alasdair Ivens; Audrey Fraser; Marie-Adele Rajandream; Tim Carver; Halina Norbertczak; Tracey Chillingworth; Zahra Hance; Kay Jagels; Sharon Moule; Doug Ormond; Simon Rutter; Rob Squares; Sally Whitehead; Ester Rabbinowitsch; Claire Arrowsmith; Brian White; Scott Thurston; Frédéric Bringaud; Sandra L Baldauf; Adam Faulconbridge; Daniel Jeffares; Daniel P Depledge; Samuel O Oyola; James D Hilley; Loislene O Brito; Luiz R O Tosi; Barclay Barrell; Angela K Cruz; Jeremy C Mottram; Deborah F Smith; Matthew Berriman Journal: Nat Genet Date: 2007-06-17 Impact factor: 38.330