Literature DB >> 3364723

A radioisotopic assay of picomolar concentrations of coenzyme A in liver tissue.

K M Knights1, R Drew.   

Abstract

A single-step enzyme assay using [14C]palmitic acid and bacterial acyl-coenzyme A synthetase (EC 6.2.1.3) is described for the determination of reduced coenzyme A (CoASH) levels in liver samples. Use of this technique provides a rapid and accurate determination of CoASH in the range 1-250 pmol. Application of the method to the quantitation of CoASH in samples of human liver tissue and rat liver homogenate, isolated hepatocytes, and mitochondria is described.

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Year:  1988        PMID： 3364723     DOI： 10.1016/0003-2697(88)90015-2

Source DB:  PubMed          Journal:  Anal Biochem        ISSN： 0003-2697            Impact factor:   3.365


  3 in total

1.  Plant coenzyme A biosynthesis: characterization of two pantothenate kinases from Arabidopsis.

Authors:  G B Tilton; W J Wedemeyer; J Browse; J Ohlrogge
Journal:  Plant Mol Biol       Date:  2006-07       Impact factor: 4.076

2.  Chemical knockout of pantothenate kinase reveals the metabolic and genetic program responsible for hepatic coenzyme A homeostasis.

Authors:  Yong-Mei Zhang; Shigeru Chohnan; Kristopher G Virga; Robert D Stevens; Olga R Ilkayeva; Brett R Wenner; James R Bain; Christopher B Newgard; Richard E Lee; Charles O Rock; Suzanne Jackowski
Journal:  Chem Biol       Date:  2007-03

3.  Mitochondrial, but not peroxisomal, beta-oxidation of fatty acids is conserved in coenzyme A-deficient rat liver.

Authors:  J A Youssef; W O Song; M Z Badr
Journal:  Mol Cell Biochem       Date:  1997-10       Impact factor: 3.396
  3 in total

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