Literature DB >> 3364706

Fast atom bombardment-mass spectrometry of glycosphingolipids extracted from thin-layer chromatography plates.

P Påhlsson1, B Nilsson.   

Abstract

A method is described for analysis of glycosphingolipids extracted from thin-layer chromatography plates. Mixtures of glycolipids and gangliosides were separated by thin-layer chromatography and the individual bands were eluted, permethylated, and, after purification, analyzed by fast atom bombardment-mass spectrometry. The glycosphingolipids could be characterized from their fast atom bombardment mass spectra in terms of partial monosaccharide sequence, ceramide composition, and molecular weight. The sensitivity of the method allows characterization of 1-5 micrograms of glycosphingolipid.

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Year:  1988        PMID： 3364706     DOI： 10.1016/0003-2697(88)90018-8

Source DB:  PubMed          Journal:  Anal Biochem        ISSN： 0003-2697            Impact factor:   3.365


  8 in total

Review 1.  Sphingolipidomics: methods for the comprehensive analysis of sphingolipids.

Authors:  Christopher A Haynes; Jeremy C Allegood; Hyejung Park; M Cameron Sullards
Journal:  J Chromatogr B Analyt Technol Biomed Life Sci       Date:  2008-12-31       Impact factor: 3.205

2.  Lipidomics of intact mitochondria by MALDI-TOF/MS.

Authors:  Roberto Angelini; Rita Vitale; Vinay A Patil; Tiziana Cocco; Bernd Ludwig; Miriam L Greenberg; Angela Corcelli
Journal:  J Lipid Res       Date:  2012-05-03       Impact factor: 5.922

3.  Glycosphingolipids of human urothelial cell lines with different grades of transformation.

Authors:  M Ugorski; P Påhlsson; D Dus; B Nilsson; C Radzikowski
Journal:  Glycoconj J       Date:  1989       Impact factor: 2.916

4.  Screening for the presence of polyglycosylceramides in various tissues: partial characterization of blood group-active complex glycosphingolipids of rabbit and dog small intestines.

Authors:  H Miller-Podraza; G Stenhagen; T Larsson; C Andersson; K A Karlsson
Journal:  Glycoconj J       Date:  1997-02       Impact factor: 2.916

5.  Carbohydrate antigen expression in murine embryonic stem cells and embryos. II. Sialylated antigens and glycolipid analysis.

Authors:  S J Kimber; D G Brown; P Påhlsson; B Nilsson
Journal:  Histochem J       Date:  1993-09

6.  Matrix-assisted laser desorption/ionization time-of-flight mass spectrometric analysis of cellular glycerophospholipids enabled by multiplexed solvent dependent analyte-matrix interactions.

Authors:  Gang Sun; Kui Yang; Zhongdan Zhao; Shaoping Guan; Xianlin Han; Richard W Gross
Journal:  Anal Chem       Date:  2008-09-04       Impact factor: 6.986

7.  Retrograde transport from the Golgi complex to the ER of both Shiga toxin and the nontoxic Shiga B-fragment is regulated by butyric acid and cAMP.

Authors:  K Sandvig; M Ryd; O Garred; E Schweda; P K Holm; B van Deurs
Journal:  J Cell Biol       Date:  1994-07       Impact factor: 10.539

8.  High-energy collision-induced dissociation of ceramide ions from permethylated glycosphingolipids.

Authors:  D D Ju; G J Wei; G R Her
Journal:  J Am Soc Mass Spectrom       Date:  1994-06       Impact factor: 3.109
  8 in total

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