Saber Gaderpour1, Rafighe Ghiasi2,1, Golamreza Hamidian3, Hamed Heydari1, Rana Keyhanmanesh2,1,4. 1. Department of Physiology, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran. 2. Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran. 3. Department of Basic Sciences, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran. 4. Neurogenic Inflammation Research Center, Mashhad University of Medical Sciences, Mashhad, Iran.
Abstract
OBJECTIVES: This research was designed to demonstrate the impact of voluntary exercise on sperm parameters including sperm count, morphology, motility, viability, testicular apoptosis, oxidative stress, and the mir-34a/SIRT1/p53 pathway in type 2 diabetic rats. MATERIALS AND METHODS: 32 Wistar male rats were separated into four groups: control (C), voluntary exercise (VE), diabetic (D), and diabetic rats that performed voluntary exercise (VED). To induce diabetes, animals were injected with streptozotocin (35 mg/kg) after receiving a high-fat diet. The testicular protein levels of SIRT1 and P53, miR-34a expression, MDA, GPx, SOD, catalase, and sperm parameters were evaluated. RESULTS: Diabetes caused increased testicular MDA content, miR-34a expression, acetylated p53 protein expression, and the percent of immotile sperm (P<0.01 to P<0.001) as well as reduced testicular GPx, SOD and catalase activities, SIRT1 protein expression, and sperm parameters (P<0.05 to P<0.001). Voluntary exercise reduced testicular MDA content, miR-34a, and acetylated p53 protein expression compared with the D group (P<0.001), however, GPx, SOD, catalase activities, and sperm parameters in voluntarily exercised rats were elevated compared with diabetic rats (P<0.05 to P<0.001). CONCLUSION: It seems that voluntary exercise has significant positive impacts that can be employed to reduce the complications of type 2 diabetes in the testis of male rats.
OBJECTIVES: This research was designed to demonstrate the impact of voluntary exercise on sperm parameters including sperm count, morphology, motility, viability, testicular apoptosis, oxidative stress, and the mir-34a/SIRT1/p53 pathway in type 2 diabetic rats. MATERIALS AND METHODS: 32 Wistar male rats were separated into four groups: control (C), voluntary exercise (VE), diabetic (D), and diabetic rats that performed voluntary exercise (VED). To induce diabetes, animals were injected with streptozotocin (35 mg/kg) after receiving a high-fat diet. The testicular protein levels of SIRT1 and P53, miR-34a expression, MDA, GPx, SOD, catalase, and sperm parameters were evaluated. RESULTS: Diabetes caused increased testicular MDA content, miR-34a expression, acetylated p53 protein expression, and the percent of immotile sperm (P<0.01 to P<0.001) as well as reduced testicular GPx, SOD and catalase activities, SIRT1 protein expression, and sperm parameters (P<0.05 to P<0.001). Voluntary exercise reduced testicular MDA content, miR-34a, and acetylated p53 protein expression compared with the D group (P<0.001), however, GPx, SOD, catalase activities, and sperm parameters in voluntarily exercised rats were elevated compared with diabetic rats (P<0.05 to P<0.001). CONCLUSION: It seems that voluntary exercise has significant positive impacts that can be employed to reduce the complications of type 2 diabetes in the testis of male rats.