| Literature DB >> 33640990 |
Anwar Sheed Khan1, Muhammad Tahir Khan2, Sajid Ali5, Taj Ali Khan3, Muhammad Qasim3, Arif Malik4, Sajid Ali5, Wasim Sajjad6, Qurrat Ul Ain7, Muhammad Irfan8.
Abstract
Regardless of a plethora of advanced diagnostics, TB and drug resistance remains a principal killer. We proposed gold nanoparticles (AuNPs) attached with probes to enhance the efficiency of GeneXpert MTB/RIF assay instead of conventional dye probes for molecular detection. A total of 15,000 samples were collected from TB suspects and subjected to Xpert MTB/RIF assay, where 6800 (45.3%) were detected as MTB positive, 280 (4.3%) were detected to harbor mutations in the RRDR, while invalid /errors were found in 690 (4.6%) cases. The mutations were detected by probe E, 199 (71.1%), while probes B and D, 30 and 26 (10% and 9%), respectively. In the Xpert MTB/RIF Assay were found mutations picked by probes E and B codons 529-533 (71%) and 512-518 (10%), respectively. The fast-rising works of TB nano-diagnostics, of Xpert probes, may improve by the applications of gold nanoparticle probes.Entities:
Keywords: AuNPs; Probe; RRDR; Xpert MTB/RIF assay
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Year: 2021 PMID： 33640990 DOI： 10.1007/s00203-021-02242-5
Source DB: PubMed Journal: Arch Microbiol ISSN： 0302-8933 Impact factor: 2.552