Guangqin Cai1,2,3,4, Geliang Wang5,6, Sang-Chul Kim5,6, Jianwu Li5,6, Yongming Zhou7, Xuemin Wang8,9. 1. Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture and Rural Affairs, Oil Crop Research Institute, Chinese Academy of Agricultural Sciences, Wuhan, 430062, Hubei, China. cgq6688@163.com. 2. National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, 430070, Hubei, China. cgq6688@163.com. 3. Department of Biology, University of Missouri, St. Louis, MO, 63121, USA. cgq6688@163.com. 4. Donald Danforth Plant Science Center, St. Louis, MO, 63132, USA. cgq6688@163.com. 5. Department of Biology, University of Missouri, St. Louis, MO, 63121, USA. 6. Donald Danforth Plant Science Center, St. Louis, MO, 63132, USA. 7. National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, 430070, Hubei, China. 8. Department of Biology, University of Missouri, St. Louis, MO, 63121, USA. swang@danforthcenter.org. 9. Donald Danforth Plant Science Center, St. Louis, MO, 63132, USA. swang@danforthcenter.org.
Abstract
BACKGROUND: Lipid transporters play an essential role in lipid delivery and distribution, but their influence on seed oil production in oilseed crops is not well studied. RESULTS: Here, we examined the effect of two lipid transporters, FAX1 (fatty acid export1) and ABCA9 (ATP-binding cassette transporter subfamily A9) on oil production and lipid metabolism in the oilseed plant Camelina sativa. Overexpression (OE) of FAX1 and ABCA9 increased seed weight and size, with FAX1-OEs and ABCA9-OEs increasing seed length and width, respectively, whereas FAX1/ABCA9-OEs increasing both. FAX1-OE and ABCA9-OE displayed additive effects on seed oil content and seed yield. Also, OE of FAX1 and ABCA9 affected membrane lipid composition in developing pods, especially on phosphatidylcholine, phosphatidylethanolamine, and phosphatidylglycerol. The expression of some genes involved in seed oil synthesis, such as DGAT2, PDAT1, and LEC1, was increased in developing seeds of FAX1- and/or ABCA9-OEs. CONCLUSION: These results indicate that increased expression of FAX1 and ABCA9 can potentially be applied to improving camelina oil production.
BACKGROUND:Lipid transporters play an essential role in lipid delivery and distribution, but their influence on seed oil production in oilseed crops is not well studied. RESULTS: Here, we examined the effect of two lipid transporters, FAX1 (fatty acid export1) and ABCA9 (ATP-binding cassette transporter subfamily A9) on oil production and lipid metabolism in the oilseed plant Camelina sativa. Overexpression (OE) of FAX1 and ABCA9 increased seed weight and size, with FAX1-OEs and ABCA9-OEs increasing seed length and width, respectively, whereas FAX1/ABCA9-OEs increasing both. FAX1-OE and ABCA9-OE displayed additive effects on seed oil content and seed yield. Also, OE of FAX1 and ABCA9 affected membrane lipid composition in developing pods, especially on phosphatidylcholine, phosphatidylethanolamine, and phosphatidylglycerol. The expression of some genes involved in seed oil synthesis, such as DGAT2, PDAT1, and LEC1, was increased in developing seeds of FAX1- and/or ABCA9-OEs. CONCLUSION: These results indicate that increased expression of FAX1 and ABCA9 can potentially be applied to improving camelinaoil production.
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