Jingyi Yu1, Lulin Rao1, Lingling Zhan1, Bingjie Wang2, Qing Zhan3, Yanlei Xu3, Huilin Zhao2, Xinyi Wang2, Yan Zhou4, Yinjuan Guo2,5, Xiaocui Wu2,5, Zengqiang Song6, Fangyou Yu7,8. 1. Department of Laboratory Medicine, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, 325000, China. 2. Department of Clinical Laboratory, Shanghai Pulmonary Hospital, Tongji University School of Medicine, Shanghai, 200082, China. 3. Nanchang University, Nanchang, 330027, China. 4. School of Pharmaceutical Sciences, Wenzhou Medical University, Wenzhou, 325000, China. 5. Shanghai Key Laboratory of Tuberculosis, Shanghai Pulmonary Hospital, Tongji University School of Medicine, Shanghai, 200082, China. 6. School of Pharmaceutical Sciences, Wenzhou Medical University, Wenzhou, 325000, China. songzengqiang09@163.com. 7. Department of Clinical Laboratory, Shanghai Pulmonary Hospital, Tongji University School of Medicine, Shanghai, 200082, China. wzjxyfy@163.com. 8. Shanghai Key Laboratory of Tuberculosis, Shanghai Pulmonary Hospital, Tongji University School of Medicine, Shanghai, 200082, China. wzjxyfy@163.com.
Abstract
BACKGROUND: In recent years, clinical Staphylococcus aureus isolates have become highly resistant to antibiotics, which has raised concerns about the ability to control infections by these organisms. The aim of this study was to clarify the effect of a new small molecule, ZY-214-4 (C19H11BrNO4), on S. aureus pigment production. RESULTS: At the concentration of 4 μg/mL, ZY-214-4 exerted a significant inhibitory effect on S. aureus pigment synthesis, without affecting its growth or inducing a toxic effect on the silkworm. An oxidant sensitivity test and a whole-blood killing test indicated that the S. aureus survival rate decreased significantly with ZY-214-4 treatment. Additionally, ZY-214-4 administration significantly reduced the expression of a pigment synthesis-related gene (crtM) and the superoxide dismutase genes (sodA) as determined by real-time quantitative polymerase chain reaction (RT-qPCR) analysis. ZY-214-4 treatment also improved the survival rate of S. aureus-infected silkworm larvae. CONCLUSIONS: The small molecule ZY-214-4 has potential for the prevention of S. aureus infections by reducing the virulence associated with this bacterium.
BACKGROUND: In recent years, clinical Staphylococcus aureus isolates have become highly resistant to antibiotics, which has raised concerns about the ability to control infections by these organisms. The aim of this study was to clarify the effect of a new small molecule, ZY-214-4 (C19H11BrNO4), on S. aureus pigment production. RESULTS: At the concentration of 4 μg/mL, ZY-214-4 exerted a significant inhibitory effect on S. aureus pigment synthesis, without affecting its growth or inducing a toxic effect on the silkworm. An oxidant sensitivity test and a whole-blood killing test indicated that the S. aureus survival rate decreased significantly with ZY-214-4 treatment. Additionally, ZY-214-4 administration significantly reduced the expression of a pigment synthesis-related gene (crtM) and the superoxide dismutase genes (sodA) as determined by real-time quantitative polymerase chain reaction (RT-qPCR) analysis. ZY-214-4 treatment also improved the survival rate of S. aureus-infectedsilkworm larvae. CONCLUSIONS: The small molecule ZY-214-4 has potential for the prevention of S. aureus infections by reducing the virulence associated with this bacterium.
Authors: Steven Y C Tong; Joshua S Davis; Emily Eichenberger; Thomas L Holland; Vance G Fowler Journal: Clin Microbiol Rev Date: 2015-07 Impact factor: 26.132
Authors: Chia-I Liu; George Y Liu; Yongcheng Song; Fenglin Yin; Mary E Hensler; Wen-Yih Jeng; Victor Nizet; Andrew H-J Wang; Eric Oldfield Journal: Science Date: 2008-02-14 Impact factor: 47.728
Authors: Rachel J Gorwitz; Deanna Kruszon-Moran; Sigrid K McAllister; Geraldine McQuillan; Linda K McDougal; Gregory E Fosheim; Bette J Jensen; George Killgore; Fred C Tenover; Matthew J Kuehnert Journal: J Infect Dis Date: 2008-05-01 Impact factor: 5.226