| Literature DB >> 33638993 |
Dan Shen1,2, Chengyi Song1, Csaba Miskey2, Shuheng Chan1, Zhongxia Guan1, Yatong Sang1, Yali Wang1, Cai Chen1, Xiaoyan Wang1, Ferenc Müller3, Zoltán Ivics2, Bo Gao1.
Abstract
New genetic tools and strategies are currently under development to facilitate functional genomics analyses. Here, we describe an active member of the Tc1/mariner transposon superfamily, named ZB, which invaded the zebrafish genome very recently. ZB exhibits high activity in vertebrate cells, in the range of those of the widely used transposons piggyBac (PB), Sleeping Beauty (SB) and Tol2. ZB has a similar structural organization and target site sequence preference to SB, but a different integration profile with respect to genome-wide preference among mammalian functional annotation features. Namely, ZB displays a preference for integration into transcriptional regulatory regions of genes. Accordingly, we demonstrate the utility of ZB for enhancer trapping in zebrafish embryos and in the mouse germline. These results indicate that ZB may be a powerful tool for genetic manipulation in vertebrate model species.Entities:
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Year: 2021 PMID： 33638993 PMCID： PMC7913693 DOI： 10.1093/nar/gkab045
Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971