Yin Wang1,2, Yusha Meng3,4, Yanping Ma3,4, Lei Liu3,4, Dianxing Wu5, Xiaoli Shu5, Liqing Pan6, Qixian Lai3,4. 1. Institute of Rural Development, Zhejiang Academy of Agricultural Sciences, Hangzhou, 310021, China. wangyin@zaas.ac.cn. 2. Key Laboratory of Creative Agriculture, Ministry of Agriculture, Hangzhou, 310021, China. wangyin@zaas.ac.cn. 3. Institute of Rural Development, Zhejiang Academy of Agricultural Sciences, Hangzhou, 310021, China. 4. Key Laboratory of Creative Agriculture, Ministry of Agriculture, Hangzhou, 310021, China. 5. State Key Laboratory of Rice Biology, Institute of Nuclear Agriculture Sciences, Zhejiang University, Hangzhou, 310029, China. 6. Yuyao County Agricultural Techniques Promotion and Service Station, Yuyao Agricultural and Rural Bureau, Ningbo, 315400, China.
Abstract
BACKGROUND: Zn deficiency is one of the leading public health problems in the world. Staple food crop, such as rice, cannot provide enough Zn to meet the daily dietary requirement because Zn in grain would chelate with phytic acid, which resulted in low Zn bioavailability. Breeding new rice varieties with high Zn bioavailability will be an effective, economic and sustainable strategy to alleviate human Zn deficiency. RESULTS: The high Zn density mutant LLZ was crossed with the low phytic acid mutant Os-lpa-XS110-1, and the contents of Zn and phytic acid in the brown rice were determined for the resulting progenies grown at different sites. Among the hybrid progenies, the double mutant always displayed significantly higher Zn content and lower phytic acid content in grain, leading to the lowest molar ratio of phytic acid to Zn under all environments. As assessed by in vitro digestion/Caco-2 cell model, the double mutant contained the relatively high content of bioavailable Zn in brown rice. CONCLUSIONS: Our findings suggested pyramiding breeding by a combination of high Zn density and low phytic acid is a practical and useful approach to improve Zn bioavailability in rice grain.
BACKGROUND:pan class="Chemical">Zndeficiency is one of the leading public health problems in the world. Staple food crop, such as rice, cannot provide enough Zn to meet the daily dietary requirement because Zn in grain would chelate with phytic acid, which resulted in low Zn bioavailability. Breeding new rice varieties with high Zn bioavailability will be an effective, economic and sustainable strategy to alleviate humanZndeficiency. RESULTS: The high Zn density mutant LLZ was crossed with the low phytic acid mutant Os-lpa-XS110-1, and the contents of Zn and phytic acid in the brown rice were determined for the resulting progenies grown at different sites. Among the hybrid progenies, the double mutant always displayed significantly higher Zn content and lower phytic acid content in grain, leading to the lowest molar ratio of phytic acid to Zn under all environments. As assessed by in vitro digestion/Caco-2 cell model, the double mutant contained the relatively high content of bioavailable Zn in brown rice. CONCLUSIONS: Our findings suggested pyramiding breeding by a combination of high Zn density and low phytic acid is a practical and useful approach to improve Zn bioavailability in rice grain.
Authors: Alexander A T Johnson; Bianca Kyriacou; Damien L Callahan; Lorraine Carruthers; James Stangoulis; Enzo Lombi; Mark Tester Journal: PLoS One Date: 2011-09-06 Impact factor: 3.240
Authors: Somayanda M Impa; Mark J Morete; Abdelbagi M Ismail; Rainer Schulin; Sarah E Johnson-Beebout Journal: J Exp Bot Date: 2013-05-22 Impact factor: 6.992