Funda Çağırır Dindaroğlu1,2, Nesrin Eronat3, Asude Durmaz4, Dilşah Çoğulu3, Burak Durmaz4, Özgür Çoğulu4. 1. Department of Pediatric Dentistry, School of Dentistry, Ege University, Izmir, Turkey. fundacagirir@gmail.com. 2. Department of Pediatric Dentistry, School of Dentistry, İzmir Katip Çelebi University, Izmir, Turkey. fundacagirir@gmail.com. 3. Department of Pediatric Dentistry, School of Dentistry, Ege University, Izmir, Turkey. 4. Department of Medical Genetics, School of Medicine, Ege University, Izmir, Turkey.
Abstract
OBJECTIVES: The variation in the caries susceptibility while environmental factors are similar indicates that the effect of individual factors such as genetics on caries process and tooth development should be revealed. The aim of this study was to evaluate the association between genetic polymorphisms in MMP13 (rs2252070) and MMP20 (rs1784418) with caries experience. MATERIALS AND METHODS: A cross-sectional study was conducted on 200 subjects aged 6 to 14 years. Demographic data, data on oral health habits were obtained through the statements of guardian of the individuals, caries data was collected by clinical examination. Unstimulated whole saliva was collected to extract the genomic DNA. Genotyping of the selected polymorphisms was carried out by real-time PCR. Allele and genotype frequencies were compared between different subgroups considering caries experience. Data were analyzed using SPSS 16.0 by chi-square test and logistic regression analysis. RESULTS: Allele distribution of MMP13 was different between caries-affected and caries-free subjects. MMP13 A allele increased the caries risk (p=0.005, OR=1.84, 95% CI 1.20-2.82). Allele and genotype distribution of the polymorphism in MMP20 were not associated with caries experience (p>0.05). CONCLUSIONS: It is concluded that the genetic variation in MMP13 was associated with the caries experience in selected subjects in Turkey. CLINICAL RELEVANCE: The knowledge regarding association between the MMP genes and caries experience, might benefit the clinical practice, improving caries-preventive and caries-therapeutic approaches.
OBJECTIVES: The variation in the caries susceptibility while environmental factors are similar indicates that the effect of individual factors such as genetics on caries process and tooth development should be revealed. The aim of this study was to evaluate the association between genetic polymorphisms in MMP13 (rs2252070) and MMP20 (rs1784418) with caries experience. MATERIALS AND METHODS: A cross-sectional study was conducted on 200 subjects aged 6 to 14 years. Demographic data, data on oral health habits were obtained through the statements of guardian of the individuals, caries data was collected by clinical examination. Unstimulated whole saliva was collected to extract the genomic DNA. Genotyping of the selected polymorphisms was carried out by real-time PCR. Allele and genotype frequencies were compared between different subgroups considering caries experience. Data were analyzed using SPSS 16.0 by chi-square test and logistic regression analysis. RESULTS: Allele distribution of MMP13 was different between caries-affected and caries-free subjects. MMP13 A allele increased the caries risk (p=0.005, OR=1.84, 95% CI 1.20-2.82). Allele and genotype distribution of the polymorphism in MMP20 were not associated with caries experience (p>0.05). CONCLUSIONS: It is concluded that the genetic variation in MMP13 was associated with the caries experience in selected subjects in Turkey. CLINICAL RELEVANCE: The knowledge regarding association between the MMP genes and caries experience, might benefit the clinical practice, improving caries-preventive and caries-therapeutic approaches.
Authors: P N Tannure; E C Küchler; P Falagan-Lotsch; L M F Amorim; R Raggio Luiz; M C Costa; A R Vieira; J M Granjeiro Journal: Caries Res Date: 2012-06-15 Impact factor: 4.056
Authors: M Kilian; I L C Chapple; M Hannig; P D Marsh; V Meuric; A M L Pedersen; M S Tonetti; W G Wade; E Zaura Journal: Br Dent J Date: 2016-11-18 Impact factor: 1.626
Authors: Christina J Adler; Keith Dobney; Laura S Weyrich; John Kaidonis; Alan W Walker; Wolfgang Haak; Corey J A Bradshaw; Grant Townsend; Arkadiusz Sołtysiak; Kurt W Alt; Julian Parkhill; Alan Cooper Journal: Nat Genet Date: 2013-02-17 Impact factor: 38.330
Authors: João Armando Brancher; Giovana Daniela Pecharki; Andrea Duarte Doetzer; Kamilla Gabriella Dos Santos Medeiros; Carlos Alberto Cordeiro Júnior; Vanessa Santos Sotomaior; Peter Bauer; Paula Cristina Trevilatto Journal: Int J Dent Date: 2011-12-08