Literature DB >> 336387

A procedure for the rapid freezing of whole embryos.

D F Newgreen, I J Allan.   

Abstract

A procedure for the rapid freezing of whole chick embryos for histochemical treatment is described. The problems of deformation during preparation for quenching and orientation for sectioning have been largely overcome by placing embryos inside lengths of chicken trachea. The subsequent disorientation of tissues that follows cracking and shattering due to the rapid freezing of whole embryos is avoided. The method permitted a more precise identification of the position and time of appearance of formaldehyde-induced fluorescence and myosin antibody immunofluorescence in serially sectioned embryos.

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Mesh：

Year:  1977        PMID： 336387     DOI： 10.1007/bf01918842

Source DB:  PubMed          Journal:  Experientia        ISSN： 0014-4754


  10 in total

1.  APUD cells in the endocrine pancreas and the intestine of chick embryos.

Authors:  A Andrew
Journal:  Gen Comp Endocrinol       Date:  1975-08       Impact factor: 2.822

2.  ULTRASTRUCTURE OF DEVELOPING MUSCLE CELLS IN THE CHICK EMBRYO.

Authors:  E R ALLEN; F A PEPE
Journal:  Am J Anat       Date:  1965-01

3.  A correlative histofluorescence and light microscopic study of the formation of the sympathetic trunks in chick embryos.

Authors:  M L Kirby; S A Gilmore
Journal:  Anat Rec       Date:  1976-11

4.  A series of normal stages in the development of the chick embryo.

Authors:  V HAMBURGER; H L HAMILTON
Journal:  J Morphol       Date:  1951-01       Impact factor: 1.804

5.  Antibody to myosin: the specific visualization of myosin-containing filaments in nonmuscle cells.

Authors:  K Weber; U Groeschel-Stewart
Journal:  Proc Natl Acad Sci U S A       Date:  1974-11       Impact factor: 11.205

6.  Catecholamines and morphogenesis of the chick neural tube and notochord.

Authors:  I E Lawrence; H W Burden
Journal:  Am J Anat       Date:  1973-06

7.  Sarcomere formation in chick striated muscle.

Authors:  E R Allen
Journal:  Z Zellforsch Mikrosk Anat       Date:  1973-11-29

8.  A fluorescence study on the ability of the notochord to synthesize and store catecholamines in early chick embryos.

Authors:  M L Kirby; S A Gilmore
Journal:  Anat Rec       Date:  1972-08

9.  Fluorogenic amine tracing of neural crest derivatives forming the adrenal medulla.

Authors:  J M Polak; F W Rost; A G Pearse
Journal:  Gen Comp Endocrinol       Date:  1971-02       Impact factor: 2.822

10.  Catecholamine accumulation in neural crest cells and the primary sympathetic chain.

Authors:  I J Allan; D F Newgreen
Journal:  Am J Anat       Date:  1977-07
  10 in total
  2 in total

1.  Accumulation of exogenous catecholamines in the neural tube and non-neural tissues of the early fowl embryo : Correlation with morphogenetic movements.

Authors:  D F Newgreen; I J Allan; H M Young; B R Southwell
Journal:  Wilehm Roux Arch Dev Biol       Date:  1981-11

2.  Extraneuronal effects of 6-hydroxydopamine and extraneuronal uptake of noradrenaline. In-vivo and in-vitro studies on adrenocortical cells of lizards and rats.

Authors:  K Unsicker; I J Allan; D F Newgreen
Journal:  Cell Tissue Res       Date:  1976-10-01       Impact factor: 5.249
  2 in total

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