Qi Shen1, Yue Chen2, Junwei Sun3, Qian Liu4, Chongbo Sun5. 1. Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences, Hangzhou, Zhejiang, China. 2. Institute of Horticulture, Zhejiang Academy of Agriculture Sciences, Hangzhou, Zhejiang, China. earnchen@163.com. 3. College of Modern Science and Technology, China Jiliang University, Hangzhou, 310018, China. 4. Institute of Landscape Architecture, Nanjing Forestry University, Nanjing, Jiangsu, China. 5. Institute of Horticulture, Zhejiang Academy of Agriculture Sciences, Hangzhou, Zhejiang, China.
Abstract
Cymbidium geringii has high ornamental and economic importance. Its traits, including flower shape, size, and color, are highly sought by orchid breeders. Gaining insights into the molecular basis of C. geringi flower development would accelerate genetic improvement of other orchids. Methods and Results: Here, C. goeringii RNA was purified from normal and peloric mutant flowers, and cDNA libraries constructed for Illumina sequencing. We generated 329,156,782 clean reads, integrated them, and then assembled into 236,811 unigenes averaging 595 bp long. A total of 11,992 differentially expressed genes s, of which 6119 were upregulated and 5873 downregulated, were uncovered in peloric mutant flower buds relative to normal flower buds. Kyoto Encyclopedia of Genes and Genomes enrichment assessments posited that these differentially expressed genes are associated with "Photosynthesis", "Linoleic acid metabolism", as well as "Plant hormone signal transduction" cascades. The DEGs were designated to 12 remarkably enriched GO terms, and 16 cell wall associated GO terms. The expression level of 16 determined genes were verified using RT-qPCR. Conclusions: Our gene expression data may be used to study the regulatory mechanism of flower organ development in C. geringi.
Cymbidium geringii has high ornamental and economic importance. Its traits, including flower shape, size, and color, are highly sought by orchid breeders. Gaining insights into the molecular basis of C. geringi flower development would accelerate genetic improvement of other orchids. Methods and Results: Here, C. goeringii RNA was purified from normal and peloric mutant flowers, and cDNA libraries constructed for Illumina sequencing. We generated 329,156,782 clean reads, integrated them, and then assembled into 236,811 unigenes averaging 595 bp long. A total of 11,992 differentially expressed genes s, of which 6119 were upregulated and 5873 downregulated, were uncovered in peloric mutant flower buds relative to normal flower buds. Kyoto Encyclopedia of Genes and Genomes enrichment assessments posited that these differentially expressed genes are associated with "Photosynthesis", "Linoleic acid metabolism", as well as "Plant hormone signal transduction" cascades. The DEGs were designated to 12 remarkably enriched GO terms, and 16 cell wall associated GO terms. The expression level of 16 determined genes were verified using RT-qPCR. Conclusions: Our gene expression data may be used to study the regulatory mechanism of flower organ development in C. geringi.
Authors: Ksenia V Krasileva; Hans A Vasquez-Gross; Tyson Howell; Paul Bailey; Francine Paraiso; Leah Clissold; James Simmonds; Ricardo H Ramirez-Gonzalez; Xiaodong Wang; Philippa Borrill; Christine Fosker; Sarah Ayling; Andrew L Phillips; Cristobal Uauy; Jorge Dubcovsky Journal: Proc Natl Acad Sci U S A Date: 2017-01-17 Impact factor: 11.205