Kate Watson1, Irving Korman2, Yanhe Liu2,3,4, Deirdre Zander-Fox2,5,6,7. 1. Monash IVF Gold Coast, Level 3, 2 Short St, Southport, Queensland, 4215, Australia. kwatson@monashivf.com. 2. Monash IVF Gold Coast, Level 3, 2 Short St, Southport, Queensland, 4215, Australia. 3. School of Human Sciences, University of Western Australia, Perth, Western Australia, Australia. 4. School of Medical and Health Sciences, Edith Cowan University, Joondalup, Western Australia, Australia. 5. Department of Obstetrics and Gynecology, University of Adelaide, Adelaide, South Australia, Australia. 6. Department of Bioengineering, University of South Australia, Adelaide, South Australia, Australia. 7. Monash University, Monash, Victoria, Australia.
Abstract
OBJECTIVE: To report a live birth from a patient with complete zona-free oocytes due to abnormal zona production and to reveal full time-lapse blastocyst development footage of its originating embryo. METHODS: A 34-year-old woman presented with a history of failed fertilization via standard in vitro fertilization insemination and a potential absence of zona pellucida. A total of 3 intracytoplasmic sperm injection cycles were undertaken with all oocytes collected being zona-free. Embryos created in the initial 2 cycles were cultured in the G1+/G2+ sequential media in a benchtop incubator. During the final successful cycle, the culture strategy was shifted to single step media (G-TL) in an Embryoscope+ incubator. RESULTS: The first 2 attempts led to a biochemical pregnancy or no blastocyst available for transfer. In the third cycle, 13 out of 24 collected oocytes were subjected to injection, with 4 being normally fertilized. Two blastocysts were subsequently formed, in which one was cryopreserved and the other transferred. A live baby girl (1570g) was subsequently delivered at 34 weeks of gestation by cesarean section. CONCLUSION: Live birth can be achieved for patients with zona production deficiency. Adjustment in ovarian stimulation and subsequent embryo culture strategies may have potentially contributed to the success of the 3rd cycle.
OBJECTIVE: To report a live birth from a patient with complete zona-free oocytes due to abnormal zona production and to reveal full time-lapse blastocyst development footage of its originating embryo. METHODS: A 34-year-old woman presented with a history of failed fertilization via standard in vitro fertilization insemination and a potential absence of zona pellucida. A total of 3 intracytoplasmic sperm injection cycles were undertaken with all oocytes collected being zona-free. Embryos created in the initial 2 cycles were cultured in the G1+/G2+ sequential media in a benchtop incubator. During the final successful cycle, the culture strategy was shifted to single step media (G-TL) in an Embryoscope+ incubator. RESULTS: The first 2 attempts led to a biochemical pregnancy or no blastocyst available for transfer. In the third cycle, 13 out of 24 collected oocytes were subjected to injection, with 4 being normally fertilized. Two blastocysts were subsequently formed, in which one was cryopreserved and the other transferred. A live baby girl (1570g) was subsequently delivered at 34 weeks of gestation by cesarean section. CONCLUSION: Live birth can be achieved for patients with zona production deficiency. Adjustment in ovarian stimulation and subsequent embryo culture strategies may have potentially contributed to the success of the 3rd cycle.
Entities:
Keywords:
Case report; Live birth; Single stage culture; Time-lapse; Zona-free
Authors: L Lefièvre; S J Conner; A Salpekar; O Olufowobi; P Ashton; B Pavlovic; W Lenton; M Afnan; I A Brewis; M Monk; D C Hughes; C L R Barratt Journal: Hum Reprod Date: 2004-05-13 Impact factor: 6.918