Narges Marefati1, Farimah Beheshti2,3, Sara Memarpour4, Mohammad Rezaei5, Mahmoud Hosseini6. 1. Neurogenic Inflammation Research Center and Department of Physiology, School of Medicine, Mashhad University of Medical Sciences, Iran. 2. Neuroscience Research Center, Torbat Heydariyeh University of Medical Sciences, Torbat Heydariyeh, Iran. 3. Department of Physiology, School of Paramedical Sciences, Torbat Heydariyeh University of Medical Sciences, Torbat Heydariyeh, Iran. 4. Mollecular Medicine Department, Mashhad University of Medical Sciences, Mashhad, Iran. 5. Student Research Committee, Department of Physiology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran. 6. Division of Neurocognitive Sciences, Psychiatry and Behavioral Sciences Research Center, Mashhad University of Medical Sciences, Mashhad, Iran.
Abstract
OBJECTIVE: Olibanum (OLIB) and its component boswellic acid (BOSA) are suggested to have anti-inflammatory, anti-oxidant and neuroprotective effects. In the present work, we examined effect of OLIB, and BOSA on the synaptic plasticity impairment and oxidative stress indicators in a rat model of neuro-inflammation induced by lipopolysaccharide (LPS). MATERIALS AND METHODS: Forty rats were divided into the following four groups: (1) Control, (2) LPS, (3) OLIB (200 mg/kg), and (4) BOSA (10 mg/kg). The animals were pre-treated with OLIB extract, BOSA or the vehicle 30 min before LPS (1 mg/kg) administration, for 6 days. On the 6th day, electrophysiological recording was done. Long-term potentiation (LTP) from CA1 area of hippocampus was assessed. The animals were then sacrificed and their brains were removed for evaluation of the levels of interleukin-6 (IL-6), nitric oxide (NO) metabolites, malondialdehyde (MDA), thiol, superoxide dismutase (SOD) and catalase (CAT) in the cortex. RESULTS: Administration of LPS decreased amplitude (p<0.001) and slope (p<0.01) of field excitatory postsynaptic potential (fEPSP). Pre-treatment enhanced these parameters (p<0.05 to p<0.001). LPS also increased cortical levels of IL-6 (P<0.01), NO, and MDA (p<0.001) while decreased thiol, SOD (p<0.001), and CAT (p<0.05). OLIB and BOSA diminished IL-6 (p<0.05-p<0.001), NO (p<0.01-p<0.001) and MDA level (p<0.01 and p<0.001, respectively) while improved SOD (p<0.05 and p<0.001, respectively), CAT (p<0.05 and p<0.001, respectively) and thiol content (p<0.001). CONCLUSION: The results showed that OLIB and BOSA could improve synaptic plasticity impairment induced by LPS as shown by a decrease in an inflammation indicator along with the anti-oxidant effects.
OBJECTIVE: Olibanum (OLIB) and its component boswellic acid (BOSA) are suggested to have anti-inflammatory, anti-oxidant and neuroprotective effects. In the present work, we examined effect of OLIB, and BOSA on the synaptic plasticity impairment and oxidative stress indicators in a rat model of neuro-inflammation induced by lipopolysaccharide (LPS). MATERIALS AND METHODS: Forty rats were divided into the following four groups: (1) Control, (2) LPS, (3) OLIB (200 mg/kg), and (4) BOSA (10 mg/kg). The animals were pre-treated with OLIB extract, BOSA or the vehicle 30 min before LPS (1 mg/kg) administration, for 6 days. On the 6th day, electrophysiological recording was done. Long-term potentiation (LTP) from CA1 area of hippocampus was assessed. The animals were then sacrificed and their brains were removed for evaluation of the levels of interleukin-6 (IL-6), nitric oxide (NO) metabolites, malondialdehyde (MDA), thiol, superoxide dismutase (SOD) and catalase (CAT) in the cortex. RESULTS: Administration of LPS decreased amplitude (p<0.001) and slope (p<0.01) of field excitatory postsynaptic potential (fEPSP). Pre-treatment enhanced these parameters (p<0.05 to p<0.001). LPS also increased cortical levels of IL-6 (P<0.01), NO, and MDA (p<0.001) while decreased thiol, SOD (p<0.001), and CAT (p<0.05). OLIB and BOSA diminished IL-6 (p<0.05-p<0.001), NO (p<0.01-p<0.001) and MDA level (p<0.01 and p<0.001, respectively) while improved SOD (p<0.05 and p<0.001, respectively), CAT (p<0.05 and p<0.001, respectively) and thiol content (p<0.001). CONCLUSION: The results showed that OLIB and BOSA could improve synaptic plasticity impairment induced by LPS as shown by a decrease in an inflammation indicator along with the anti-oxidant effects.
Authors: D Koppenhöfer; F Kettenbaum; A Susloparova; J K Y Law; X T Vu; T Schwab; K H Schäfer; S Ingebrandt Journal: Biosens Bioelectron Date: 2014-09-08 Impact factor: 10.618