Literature DB >> 3362869

Simultaneous demonstrations of neuropeptide Y gene expression and peptide storage in single neurons of the human brain.

V Chan-Palay1, G Yasargil, Q Hamid, J M Polak, S L Palay.   

Abstract

A combination of in situ hybridization for neuropeptide Y mRNA that used a 32P-labeled complementary RNA probe and immunocytochemistry with polyclonal antibodies against neuropeptide Y were applied to human cortical brain samples to simultaneously localize neuropeptide Y and its mRNA. These two techniques allowed simultaneous identification of neuropeptide Y gene expression and peptide storage in single neurons of the human brain.

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Year:  1988        PMID： 3362869      PMCID： PMC280174          DOI： 10.1073/pnas.85.9.3213

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  14 in total

1.  Localization of neuropeptide Y mRNA in neurons of human cerebral cortex by means of in situ hybridization with a complementary RNA probe.

Authors:  G Terenghi; J M Polak; Q Hamid; E O'Brien; P Denny; S Legon; J Dixon; C D Minth; S L Palay; G Yasargil
Journal:  Proc Natl Acad Sci U S A       Date:  1987-10       Impact factor: 11.205

2.  Combination of immunocytochemistry and in situ hybridization in the same tissue section of rat pituitary.

Authors:  B D Shivers; R E Harlan; D W Pfaff; B S Schachter
Journal:  J Histochem Cytochem       Date:  1986-01       Impact factor: 2.479

3.  A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity.

Authors:  A P Feinberg; B Vogelstein
Journal:  Anal Biochem       Date:  1983-07-01       Impact factor: 3.365

4.  Cloning, characterization, and DNA sequence of a human cDNA encoding neuropeptide tyrosine.

Authors:  C D Minth; S R Bloom; J M Polak; J E Dixon
Journal:  Proc Natl Acad Sci U S A       Date:  1984-07       Impact factor: 11.205

5.  Neuropeptide Y: complete amino acid sequence of the brain peptide.

Authors:  K Tatemoto
Journal:  Proc Natl Acad Sci U S A       Date:  1982-09       Impact factor: 11.205

6.  Cortical neurons immunoreactive with antisera against neuropeptide Y are altered in Alzheimer's-type dementia.

Authors:  V Chan-Palay; W Lang; Y S Allen; U Haesler; J M Polak
Journal:  J Comp Neurol       Date:  1985-08-22       Impact factor: 3.215

7.  Cytology and distribution in normal human cerebral cortex of neurons immunoreactive with antisera against neuropeptide Y.

Authors:  V Chan-Palay; Y S Allen; W Lang; U Haesler; J M Polak
Journal:  J Comp Neurol       Date:  1985-08-22       Impact factor: 3.215

8.  Neuropeptide Y-immunoreactive nerves in the uvea of guinea pig and rat.

Authors:  G Terenghi; J M Polak; J M Allen; S Q Zhang; W G Unger; S R Bloom
Journal:  Neurosci Lett       Date:  1983-11-21       Impact factor: 3.046

9.  Neuropeptide Y distribution in the rat brain.

Authors:  Y S Allen; T E Adrian; J M Allen; K Tatemoto; T J Crow; S R Bloom; J M Polak
Journal:  Science       Date:  1983-08-26       Impact factor: 47.728

10.  Nerve fibers in the gut and pancreas of the rat displaying neuropeptide-Y immunoreactivity. Intrinsic and extrinsic origin.

Authors:  F Sundler; E Moghimzadeh; R Håkanson; M Ekelund; P Emson
Journal:  Cell Tissue Res       Date:  1983       Impact factor: 5.249
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  5 in total

1.  Localization of the mRNA for the dopamine D2 receptor in the rat brain by in situ hybridization histochemistry.

Authors:  G Mengod; M I Martinez-Mir; M T Vilaró; J M Palacios
Journal:  Proc Natl Acad Sci U S A       Date:  1989-11       Impact factor: 11.205

2.  In situ hybridization study of neuropeptide Y neurons in the rat brain and pelvic paracervical ganglion.

Authors:  E Houdeau; P A Boyer
Journal:  Cell Tissue Res       Date:  1994-09       Impact factor: 5.249

3.  A one-day double-labelling technique for tissue specimens: immunogold-silver staining for in situ hybridization combined with alkaline phosphatase-anti-alkaline phosphatase (APAAP) immunohistochemistry for antigens.

Authors:  U Müller-Ladner; J Kriegsmann; R E Gay; S Gay
Journal:  Histochem J       Date:  1996-02

4.  Techniques for the simultaneous demonstrations of neuropeptide y gene expression and peptide storage in single neurons of the human brain.

Authors:  V Chan-Palay
Journal:  Histochemistry       Date:  1988

5.  Expression of the human neuropeptide tyrosine Y1 receptor.

Authors:  J Wharton; L Gordon; J Byrne; H Herzog; L A Selbie; K Moore; M H Sullivan; M G Elder; G Moscoso; K M Taylor
Journal:  Proc Natl Acad Sci U S A       Date:  1993-01-15       Impact factor: 11.205
  5 in total

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