Bahar Sadegh Ehdaei1, Ahmad Pirouzmand1,2, Mehdi Shabani3, Arezoo Mirzaei3, Sharareh Moghim4. 1. Department of Microbiology, Kashan University of Medical Sciences, Kashan, Iran. 2. Autoimmune Diseases Research Center, Faculty of Medicine, Kashan University of Medical Sciences, Kashan, Iran. 3. Department of Microbiology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran. 4. Department of Microbiology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran, moghim@med.mui.ac.ir.
Abstract
INTRODUCTION: Herpes simplex viruses (HSVs) are widely distributed in the human population. HSV type 1 (HSV-1) is responsible for a spectrum of diseases, ranging from gingivostomatitis to keratoconjunctivitis, and encephalitis. The HSVs establish latent infections in nerve cells, and recurrences are common. Their frequent reactivation in elderly and immunosuppressed patients causes serious health complications. OBJECTIVES: Due to the growing resistance to its main drug, acyclovir, alternative treatments with different mechanisms of action are required. MicroRNAs regulate host and viral gene expression posttranscriptionally. Previous studies reported that mir-101-2 expression has widely participated in the regulation of HSV-1 replication. In this study, we investigate the effect of hsa-miR-101-1 in the replication of HSV-1. METHODS: We found that transfection of miR-101-1 into HeLa cells could reduce effectively HSV-1 replication using plaque assay and real-time PCR methods. RESULTS: We showed that overexpression of miR-10-1 produced less viral progeny and manifested a weaker cytopathic effect, without affecting cell viability. DISCUSSION/ CONCLUSION: This result can give us new insights into the control of HSV-1 infections.
INTRODUCTION: Herpes simplex viruses (HSVs) are widely distributed in the human population. HSV type 1 (HSV-1) is responsible for a spectrum of diseases, ranging from gingivostomatitis to keratoconjunctivitis, and encephalitis. The HSVs establish latent infections in nerve cells, and recurrences are common. Their frequent reactivation in elderly and immunosuppressed patients causes serious health complications. OBJECTIVES: Due to the growing resistance to its main drug, acyclovir, alternative treatments with different mechanisms of action are required. MicroRNAs regulate host and viral gene expression posttranscriptionally. Previous studies reported that mir-101-2 expression has widely participated in the regulation of HSV-1 replication. In this study, we investigate the effect of hsa-miR-101-1 in the replication of HSV-1. METHODS: We found that transfection of miR-101-1 into HeLa cells could reduce effectively HSV-1 replication using plaque assay and real-time PCR methods. RESULTS: We showed that overexpression of miR-10-1 produced less viral progeny and manifested a weaker cytopathic effect, without affecting cell viability. DISCUSSION/ CONCLUSION: This result can give us new insights into the control of HSV-1 infections.
Authors: Sara H A Agwa; Hesham Elghazaly; Mahmoud Shawky El Meteini; Sherif M Shawky; Marwa Ali; Aya M Abd Elsamee; Safa Matbouly Sayed; Nadine Sherif; Howida M Sharaf; Mohamed A Alhadidy; Marwa Matboli Journal: Cells Date: 2021-11-09 Impact factor: 6.600