OBJECTIVE: The purpose of this study was to establish a biorepository of clinical, metabolomic, and microbiome samples from adolescents with obesity as they undergo lifestyle modification. METHODS: A total of 223 adolescents aged 10 to 18 years with BMI ≥95th percentile were enrolled, along with 71 healthy weight participants. Clinical data, fasting serum, and fecal samples were collected at repeated intervals over 6 months. Herein, the study design, data collection methods, and interim analysis-including targeted serum metabolite measurements and fecal 16S ribosomal RNA gene amplicon sequencing among adolescents with obesity (n = 27) and healthy weight controls (n = 27)-are presented. RESULTS: Adolescents with obesity have higher serum alanine aminotransferase, C-reactive protein, and glycated hemoglobin, and they have lower high-density lipoprotein cholesterol when compared with healthy weight controls. Metabolomics revealed differences in branched-chain amino acid-related metabolites. Also observed was a differential abundance of specific microbial taxa and lower species diversity among adolescents with obesity when compared with the healthy weight group. CONCLUSIONS: The Pediatric Metabolism and Microbiome Study (POMMS) biorepository is available as a shared resource. Early findings suggest evidence of a metabolic signature of obesity unique to adolescents, along with confirmation of previously reported findings that describe metabolic and microbiome markers of obesity.
OBJECTIVE: The purpose of this study was to establish a biorepository of clinical, metabolomic, and microbiome samples from adolescents with obesity as they undergo lifestyle modification. METHODS: A total of 223 adolescents aged 10 to 18 years with BMI ≥95th percentile were enrolled, along with 71 healthy weight participants. Clinical data, fasting serum, and fecal samples were collected at repeated intervals over 6 months. Herein, the study design, data collection methods, and interim analysis-including targeted serum metabolite measurements and fecal 16S ribosomal RNA gene amplicon sequencing among adolescents with obesity (n = 27) and healthy weight controls (n = 27)-are presented. RESULTS: Adolescents with obesity have higher serum alanine aminotransferase, C-reactive protein, and glycated hemoglobin, and they have lower high-density lipoprotein cholesterol when compared with healthy weight controls. Metabolomics revealed differences in branched-chain amino acid-related metabolites. Also observed was a differential abundance of specific microbial taxa and lower species diversity among adolescents with obesity when compared with the healthy weight group. CONCLUSIONS: The Pediatric Metabolism and Microbiome Study (POMMS) biorepository is available as a shared resource. Early findings suggest evidence of a metabolic signature of obesity unique to adolescents, along with confirmation of previously reported findings that describe metabolic and microbiome markers of obesity.
Authors: Justin R Ryder; Alexander M Kaizer; Todd M Jenkins; Aaron S Kelly; Thomas H Inge; Gabriel Q Shaibi Journal: Obesity (Silver Spring) Date: 2019-02 Impact factor: 5.002
Authors: Maggie A Stanislawski; Dana Dabelea; Leslie A Lange; Brandie D Wagner; Catherine A Lozupone Journal: NPJ Biofilms Microbiomes Date: 2019-07-01 Impact factor: 7.290
Authors: Benjamin D Pollock; Patrick Stuchlik; Emily W Harville; Katherine T Mills; Wan Tang; Wei Chen; Lydia A Bazzano Journal: Atherosclerosis Date: 2018-11-08 Impact factor: 5.162
Authors: David C Grossman; Kirsten Bibbins-Domingo; Susan J Curry; Michael J Barry; Karina W Davidson; Chyke A Doubeni; John W Epling; Alex R Kemper; Alex H Krist; Ann E Kurth; C Seth Landefeld; Carol M Mangione; Maureen G Phipps; Michael Silverstein; Melissa A Simon; Chien-Wen Tseng Journal: JAMA Date: 2017-06-20 Impact factor: 56.272
Authors: Christian Quast; Elmar Pruesse; Pelin Yilmaz; Jan Gerken; Timmy Schweer; Pablo Yarza; Jörg Peplies; Frank Oliver Glöckner Journal: Nucleic Acids Res Date: 2012-11-28 Impact factor: 16.971
Authors: Lilianna Suarez; Asheley C Skinner; Tracy Truong; Jessica R McCann; John F Rawls; Patrick C Seed; Sarah C Armstrong Journal: Child Obes Date: 2021-11-09 Impact factor: 2.867
Authors: Bradford Becken; Lauren Davey; Dustin R Middleton; Katherine D Mueller; Agastya Sharma; Zachary C Holmes; Eric Dallow; Brenna Remick; Gregory M Barton; Lawrence A David; Jessica R McCann; Sarah C Armstrong; Per Malkus; Raphael H Valdivia Journal: mBio Date: 2021-05-18 Impact factor: 7.867
Authors: Ismael Gomez-Martinez; R Jarrett Bliton; Keith A Breau; Michael J Czerwinski; Ian A Williamson; Jia Wen; John F Rawls; Scott T Magness Journal: Cell Mol Gastroenterol Hepatol Date: 2022-04-28