"Intracellular" GABA affects the equilibrium distribution of Cl- across the plasma membrane of a GABA acceptive neuron.

The permeability of Cl- ions through single microdissected plasma membrane from Deiters' neurons was studied by a microtechnique. In particular, the time course of the passage of 36Cl- ions from a microchamber, M1, to another one, M2, across the membrane was followed. This study was performed with or without gamma-amino-butyric acid (GABA) in the two microchambers. The results suggest that in basal conditions the high intracellular concentration normally present in these neurons, 3.3 mM (1), causes a higher permeability of Cl- in the direction inside----outside in the respect of the plasma membrane. "Extracellular" GABA, 0.1 mM, is able to abolish this imbalance in Cl- permeability in the two opposite directions. This event appears to be the basis for GABA induced hyperpolarization of these neurons.