Literature DB >> 3362292

The measurement of gamma-glutamyl hydrolase (conjugase) activity in rat brain.

D M Hartley1, S R Snodgrass, P A Bradshaw.   

Abstract

An assay using the artificial substrate, 2,4-diamino-10-methyl-pteroylglutamyl-gamma-glutamate (MTX-G1), was developed to measure gamma-glutamyl hydrolase (conjugase), which hydrolyzes folylpolyglutamates. This assay allows us to: 1) measure conjugase for the first time in rat brain and 2) measure conjugase in a reliable, sensitive and inexpensive manner. The MTX-binding assay results were compared to samples analyzed by HPLC and found to vary by only 13%. The artificial substrate, MTX-G1, had a lower rate of hydrolysis than pteroylglutamyl-gamma-glutamate (Pte-G2), 70.7 +/- 0.64 and 92.6 +/- 0.22 nmoles/hr/mg protein respectively. Conjugase was semi-purified 24 fold in H2O and found to have a pH optimum of 5.0.

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Year:  1988        PMID： 3362292     DOI： 10.1007/bf00973326

Source DB:  PubMed          Journal:  Neurochem Res        ISSN： 0364-3190            Impact factor:   3.996


  18 in total

1.  A rapid, radiochemical-ligand binding assay for methotrexate.

Authors:  B A Kamen; P L Takach; R Vatev; J D Caston
Journal:  Anal Biochem       Date:  1976-01       Impact factor: 3.365

2.  Hydrolytic cleavage of methotrexate gamma-polyglutamates by folylpolyglutamyl hydrolase derived from various tumors and normal tissues of the mouse.

Authors:  L L Samuels; L J Goutas; D G Priest; J R Piper; F M Sirotnak
Journal:  Cancer Res       Date:  1986-05       Impact factor: 12.701

3.  Radioactive assay of folic acid polyglutamate conjugase(s).

Authors:  C L Krumdieck; C M Baugh
Journal:  Anal Biochem       Date:  1970-05       Impact factor: 3.365

4.  Subcellular localization of folate turnover and folate-dependent enzyme activity in mouse brain.

Authors:  G F Carl; J D Peterson; L D McClain; W F Bridgers
Journal:  J Neurochem       Date:  1980-06       Impact factor: 5.372

5.  Solid-phase synthesis of pteroylpolyglutamates.

Authors:  C L Krumdieck; C M Baugh
Journal:  Methods Enzymol       Date:  1980       Impact factor: 1.600

6.  Pteroylpolyglutamate hydrolase from human jejunal brush borders. Purification and characterization.

Authors:  C J Chandler; T T Wang; C H Halsted
Journal:  J Biol Chem       Date:  1986-01-15       Impact factor: 5.157

7.  A multidisciplinary study of folic acid neurotoxicity: interactions with kainate binding sites and relevance to the aetiology of epilepsy.

Authors:  E Tremblay; M Berger; L Nitecka; E Cavalheiro; Y Ben-Ari
Journal:  Neuroscience       Date:  1984-06       Impact factor: 3.590

8.  Intrastriatal folic acid mimics the distant but not local brain damaging properties of kainic acid.

Authors:  J W Olney; T A Fuller; T de Gubareff; J Labruyere
Journal:  Neurosci Lett       Date:  1981-09-01       Impact factor: 3.046

9.  Isolation and characterization of pteroylpolyglutamate hydrolase from rat intestinal mucosa.

Authors:  B Elsenhans; O Ahmad; I H Rosenberg
Journal:  J Biol Chem       Date:  1984-05-25       Impact factor: 5.157

Review 10.  Pteroylpolyglutamates.

Authors:  R L Kisliuk
Journal:  Mol Cell Biochem       Date:  1981-09-25       Impact factor: 3.396
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  1 in total

1.  YB-1 binds to GluR2 mRNA and CaM1 mRNA in the brain and regulates their translational levels in an activity-dependent manner.

Authors:  Toru Tanaka; Sachiyo Ohashi; Tomoko Funakoshi; Shunsuke Kobayashi
Journal:  Cell Mol Neurobiol       Date:  2010-07-08       Impact factor: 5.046
  1 in total

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