Georgia Deliconstantinos1, Konstantina Kalodimou2,3, Christos Delidakis2,3. 1. Institute of Molecular Biology and Biotechnology, F.O.R.T.H., Heraklion, Greece; ginadeli@hotmail.com. 2. Institute of Molecular Biology and Biotechnology, F.O.R.T.H., Heraklion, Greece. 3. Department of Biology, University of Crete Heraklion, Heraklion, Greece.
Abstract
BACKGROUND/AIM: The DSL proteins, Serrate and Delta, which act as Notch receptor ligands, mediate signalling between adjacent cells, when a ligand-expressing cell binds to Notch on an adjacent receiving cell. Notch is ubiquitously expressed and DSL protein mis-expression can have devastating developmental consequences. Although transcriptional regulation of Delta and Serrate has been amply documented, we examined whether they are also regulated at the level of translation. MATERIALS AND METHODS: We generated a series of deletions to investigate the initiation codon usage for Serrate using Drosophila S2 cells. RESULTS: Serrate mRNA contains three putative ATG initiation codons spanning a 60-codon region upstream of its signal peptide; we found that each one can act as an initiation codon, however, with a different translational efficiency. CONCLUSION: Serrate expression is strictly regulated at the translational level. Copyright
BACKGROUND/AIM: The DSL proteins, Serrate and Delta, which act as Notch receptor ligands, mediate signalling between adjacent cells, when a ligand-expressing cell binds to Notch on an adjacent receiving cell. Notch is ubiquitously expressed and DSL protein mis-expression can have devastating developmental consequences. Although transcriptional regulation of Delta and Serrate has been amply documented, we examined whether they are also regulated at the level of translation. MATERIALS AND METHODS: We generated a series of deletions to investigate the initiation codon usage for Serrate using Drosophila S2 cells. RESULTS: Serrate mRNA contains three putative ATG initiation codons spanning a 60-codon region upstream of its signal peptide; we found that each one can act as an initiation codon, however, with a different translational efficiency. CONCLUSION: Serrate expression is strictly regulated at the translational level. Copyright
Authors: Björn Schwanhäusser; Dorothea Busse; Na Li; Gunnar Dittmar; Johannes Schuchhardt; Jana Wolf; Wei Chen; Matthias Selbach Journal: Nature Date: 2011-05-19 Impact factor: 49.962
Authors: Katrin Strassburger; Philipp Christoph Janiesch; Sibylle Schleich; Tatyana Koledachkina; Katharine K Miller; Katharina Haneke; Yong-Sheng Cheng; Katrin Kuechler; Georg Stoecklin; Kent E Duncan; Aurelio A Teleman Journal: Nature Date: 2014-07-06 Impact factor: 49.962