Edita Kunceviciene1, Brigita Budiene2, Alina Smalinskiene3, Alvita Vilkeviciute2, Rasa Liutkeviciene2,4. 1. Institute of Biology Systems and Genetics Research, Lithuanian University of Health Sciences, Kaunas, Lithuania; edita.kunceviciene@lsmuni.lt. 2. Department of Ophthalmology, Lithuanian University of Health Sciences, Medicine Academy, Kaunas, Lithuania. 3. Institute of Biology Systems and Genetics Research, Lithuanian University of Health Sciences, Kaunas, Lithuania. 4. Neuroscience Institute, Lithuanian University of Health Sciences, Medicine Academy, Kaunas, Lithuania.
Abstract
AIM: To investigate the association of the pair box 6 gene (PAX6) and hsa-miR-328-3p with optical density of macular pigment. MATERIALS AND METHODS: We evaluated 112 individuals (34 with moderate myopia, eight with high-degree myopia, and 70 healthy individuals). The optical density of macular pigment was measured using single-wavelength reflectometry. DNA and RNA were extracted from whole blood samples. Expression of hsa-miR-328-3p and genotyping of single-nucleotide polymorphism of PAX6 (rs662702) were performed using Applied Biosystems 7900HT real-time polymerase chain reaction system. Optical density of retinal pigment epithelial cells was evaluated using Fundus plus camera. RESULTS: In the group with myopia, with increasing ∆Ct hsa-miR-328-3p, the median optical density of the retinal pigment epithelium decreased statistically significantly (p<0.032). No statistically significant association was found between SNP rs662702 genotype variant of the PAX6 gene and the optical density of the retinal pigment epithelium. CONCLUSION: The increased expression of hsa-miR-328-3p in the blood indicates a decrease in the optical density of the retinal pigment epithelium in those with myopia. Copyright
AIM: To investigate the association of the pair box 6 gene (PAX6) and hsa-miR-328-3p with optical density of macular pigment. MATERIALS AND METHODS: We evaluated 112 individuals (34 with moderate myopia, eight with high-degree myopia, and 70 healthy individuals). The optical density of macular pigment was measured using single-wavelength reflectometry. DNA and RNA were extracted from whole blood samples. Expression of hsa-miR-328-3p and genotyping of single-nucleotide polymorphism of PAX6 (rs662702) were performed using Applied Biosystems 7900HT real-time polymerase chain reaction system. Optical density of retinal pigment epithelial cells was evaluated using Fundus plus camera. RESULTS: In the group with myopia, with increasing ∆Ct hsa-miR-328-3p, the median optical density of the retinal pigment epithelium decreased statistically significantly (p<0.032). No statistically significant association was found between SNP rs662702 genotype variant of the PAX6 gene and the optical density of the retinal pigment epithelium. CONCLUSION: The increased expression of hsa-miR-328-3p in the blood indicates a decrease in the optical density of the retinal pigment epithelium in those with myopia. Copyright
Authors: Brien A Holden; David A Wilson; Monica Jong; Padmaja Sankaridurg; Timothy R Fricke; Earl L Smith; Serge Resnikoff Journal: Community Eye Health Date: 2015