Laura Boldú1, Anna Merino2, Andrea Acevedo3, Angel Molina1, José Rodellar4. 1. Hospital Clínic de Barcelona-IDIBAPS, Haematology and Cytology Unit, CORE Laboratory, Biomedical Diagnostic Centre, Spain. 2. Hospital Clínic de Barcelona-IDIBAPS, Haematology and Cytology Unit, CORE Laboratory, Biomedical Diagnostic Centre, Spain. Electronic address: amerino@clinic.cat. 3. Hospital Clínic de Barcelona-IDIBAPS, Haematology and Cytology Unit, CORE Laboratory, Biomedical Diagnostic Centre, Spain; Technical University of Catalonia, Barcelona East Engineering School, Department of Mathematics, Spain. 4. Technical University of Catalonia, Barcelona East Engineering School, Department of Mathematics, Spain.
Abstract
BACKGROUND AND OBJECTIVES: Morphological differentiation among blasts circulating in blood in acute leukaemia is challenging. Artificial intelligence decision support systems hold substantial promise as part of clinical practise in detecting haematological malignancy. This study aims to develop a deep learning-based system to predict the diagnosis of acute leukaemia using blood cell images. METHODS: A set of 731 blood smears containing 16,450 single-cell images was analysed from 100 healthy controls, 191 patients with viral infections and 148 with acute leukaemia. Training and testing sets were arranged with 85% and 15% of these smears, respectively. To find the best architecture for acute leukaemia classification VGG16, ResNet101, DenseNet121 and SENet154 were evaluated. Fine-tuning was implemented to these pre-trained CNNs to adapt their layers to our data. Once the best architecture was chosen, a system with two modules working sequentially was configured (ALNet). The first module recognised abnormal promyelocytes among other mononuclear blood cell images, such as lymphocytes, monocytes, reactive lymphocytes and blasts. The second distinguished if blasts were myeloid or lymphoid lineage. The final strategy was to predict patients' initial diagnosis of acute leukaemia lineage using the blood smear review. ALNet was assessed with smears of the testing set. RESULTS: ALNet provided the correct diagnostic prediction of all patients with promyelocytic and myeloid leukaemia. Sensitivity, specificity and precision values of 100%, 92.3% and 93.7%, respectively, were obtained for myeloid leukaemia. Regarding lymphoid leukaemia, a sensitivity of 89% and specificity and precision values of 100% were obtained. CONCLUSIONS: ALNet is a predictive model designed with two serially connected convolutional networks. It is proposed to assist clinical pathologists in the diagnosis of acute leukaemia during the blood smear review. It has been proved to distinguish neoplastic (leukaemia) and non-neoplastic (infections) diseases, as well as recognise the leukaemia lineage.
BACKGROUND AND OBJECTIVES: Morphological differentiation among blasts circulating in blood in acute leukaemia is challenging. Artificial intelligence decision support systems hold substantial promise as part of clinical practise in detecting haematological malignancy. This study aims to develop a deep learning-based system to predict the diagnosis of acute leukaemia using blood cell images. METHODS: A set of 731 blood smears containing 16,450 single-cell images was analysed from 100 healthy controls, 191 patients with viral infections and 148 with acute leukaemia. Training and testing sets were arranged with 85% and 15% of these smears, respectively. To find the best architecture for acute leukaemia classification VGG16, ResNet101, DenseNet121 and SENet154 were evaluated. Fine-tuning was implemented to these pre-trained CNNs to adapt their layers to our data. Once the best architecture was chosen, a system with two modules working sequentially was configured (ALNet). The first module recognised abnormal promyelocytes among other mononuclear blood cell images, such as lymphocytes, monocytes, reactive lymphocytes and blasts. The second distinguished if blasts were myeloid or lymphoid lineage. The final strategy was to predict patients' initial diagnosis of acute leukaemia lineage using the blood smear review. ALNet was assessed with smears of the testing set. RESULTS: ALNet provided the correct diagnostic prediction of all patients with promyelocytic and myeloid leukaemia. Sensitivity, specificity and precision values of 100%, 92.3% and 93.7%, respectively, were obtained for myeloid leukaemia. Regarding lymphoid leukaemia, a sensitivity of 89% and specificity and precision values of 100% were obtained. CONCLUSIONS: ALNet is a predictive model designed with two serially connected convolutional networks. It is proposed to assist clinical pathologists in the diagnosis of acute leukaemia during the blood smear review. It has been proved to distinguish neoplastic (leukaemia) and non-neoplastic (infections) diseases, as well as recognise the leukaemia lineage.
Authors: José Rodellar; Kevin Barrera; Santiago Alférez; Laura Boldú; Javier Laguna; Angel Molina; Anna Merino Journal: Bioengineering (Basel) Date: 2022-05-23
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Authors: Yousra El Alaoui; Adel Elomri; Marwa Qaraqe; Regina Padmanabhan; Ruba Yasin Taha; Halima El Omri; Abdelfatteh El Omri; Omar Aboumarzouk Journal: J Med Internet Res Date: 2022-07-12 Impact factor: 7.076