Measuring and compensating for ocular longitudinal chromatic aberration.

It is well known that the eye's optics and media introduce monochromatic and chromatic aberration unique to each individual. Once monochromatic aberrations are removed with adaptive optics (AO), longitudinal chromatic aberrations (LCA) define the fidelity for multi-wavelength, high-resolution vision testing and retinal imaging. AO vision simulation systems and AO scanning laser ophthalmoscopes (AOSLOs) typically use the average population LCA to compensate for focus offsets between different wavelengths precluding fine, individualized control. The eye's LCA has been characterized extensively using either subjective (visual perception) or objective (imaging) methods. Classically, these have faced inconsistencies due to extraneous factors related to depth of focus, monochromatic aberration, and wavelength-dependent light interactions with retinal tissue. Here, we introduce a filter-based Badal LCA compensator that offers the flexibility to tune LCA for each individual eye and demonstrate its feasibility for vision testing and imaging using multiple wavelengths simultaneously. Incorporating the LCA compensator in an AOSLO allowed the first objective measurements of LCA based on confocal, multi-wavelength foveal cone images and its comparison to measures obtained subjectively. The objective LCA thus obtained was consistent with subjective estimates in the same individuals and hence resolves the prior discrepancies between them. Overall, the described approach will benefit applications in retinal imaging and vision testing where the focus of multiple wavelengths needs to be controlled independently and simultaneously.