Sha Yang1,2, Jialei Zhang1,2, Yun Geng1,2, Zhaohui Tang1,2, Jianguo Wang1,2, Feng Guo1,2, Jingjing Meng1,2, Quan Wang1,3, Shubo Wan4, Xinguo Li1,2. 1. Biotechnology Research Center, Shandong Academy of Agricultural Sciences, Ji'nan, China. 2. Scientific observation and experiment station of crop cultivation in east China, Ministry of Agriculture and Rural Affairs, Dongying, China. 3. College of Life Sciences, Shandong Normal University, Ji'nan, China. 4. Shandong Academy of Agricultural Sciences/Shandong Provincial Key Laboratory of Crop Genetic Improvement, Ecology and Physiology, Ji'nan, China.
Abstract
BACKGROUND: In China, double-seed (DS) sowing (i.e., sowing two seeds per hole) has been conventionally performed towards the erect-plant-type peanuts to increase the low germination rate due to poor seed preservation conditions. However, the corresponding within-hole plant competition usually limits the subsequent plant growth and the final yield. We developed a high-yield cultivation system of single-seed (SS) precision sowing to solve this paradox, saving 20% of seeds and increasing yields by more than 10% relative to the conventional DS sowing. METHODS: To explore the mechanisms of these two different cropping patterns in peanut yields, we conducted transcriptomic and physiological comparisons in the seeding plant leaf and root tissues between SS precision sowing and standard DS sowing treatments. RESULTS: After assembly, each library contained an average of 43 million reads and generated a total of 523,800, 338 clean reads. After GO and Kyoto Encyclopedia of Genes and Genomes pathway analysis, we found the key genes for biotic and abiotic stress showed higher expression in roots of plants grown under the SS precision sowing treatment, including genes encoding disease resistance, oxidation-reduction, hormone related, and stress response transcription factors and signaling regulation proteins. In particular, the resveratrol synthesis genes related to stress and disease resistance appeared induced in roots under the SS sowing treatment. CONCLUSION: These data indicated that Aspergillus flavus resistance and stress tolerance in roots under SS precision sowing were enhanced compared with roots under the DS sowing treatment. This work benefits the development of underground pods and thus increasing peanut yields.
BACKGROUND: In China, double-seed (DS) sowing (i.e., sowing two seeds per hole) has been conventionally performed towards the erect-plant-type peanuts to increase the low germination rate due to poor seed preservation conditions. However, the corresponding within-hole plant competition usually limits the subsequent plant growth and the final yield. We developed a high-yield cultivation system of single-seed (SS) precision sowing to solve this paradox, saving 20% of seeds and increasing yields by more than 10% relative to the conventional DS sowing. METHODS: To explore the mechanisms of these two different cropping patterns in peanut yields, we conducted transcriptomic and physiological comparisons in the seeding plant leaf and root tissues between SS precision sowing and standard DS sowing treatments. RESULTS: After assembly, each library contained an average of 43 million reads and generated a total of 523,800, 338 clean reads. After GO and Kyoto Encyclopedia of Genes and Genomes pathway analysis, we found the key genes for biotic and abiotic stress showed higher expression in roots of plants grown under the SS precision sowing treatment, including genes encoding disease resistance, oxidation-reduction, hormone related, and stress response transcription factors and signaling regulation proteins. In particular, the resveratrol synthesis genes related to stress and disease resistance appeared induced in roots under the SS sowing treatment. CONCLUSION: These data indicated that Aspergillus flavus resistance and stress tolerance in roots under SS precision sowing were enhanced compared with roots under the DS sowing treatment. This work benefits the development of underground pods and thus increasing peanut yields.
Authors: Lorin Mignolet-Spruyt; Enjun Xu; Niina Idänheimo; Frank A Hoeberichts; Per Mühlenbock; Mikael Brosché; Frank Van Breusegem; Jaakko Kangasjärvi Journal: J Exp Bot Date: 2016-03-14 Impact factor: 6.992
Authors: Atia B Amin; Kumudu N Rathnayake; Won C Yim; Travis M Garcia; Beate Wone; John C Cushman; Bernard W M Wone Journal: Front Plant Sci Date: 2019-02-22 Impact factor: 5.753