| Literature DB >> 3361130 |
M R Jadus1, G B Thurman, A Mrowca-Bastin, J R Yannelli.
Abstract
Recent data generated in our laboratory indicates that human lymphokine-activated killer (LAK) cells can be induced in several commercially available serum-free medium formulations. Interestingly, LAK cells were also generated in RPMI 1640 medium plus 1000 U/ml IL-2 without addition of human serum. However, higher levels of cytolytic activity were generally obtained in the commercial serum-free formulations. Cytotoxic activity was observed both at 3 and 7 days against a LAK-sensitive cell line (Daudi). Maximal cytolytic activity was usually produced by 7 days of in vitro culture in 1000 U/ml of interleukin-2 (IL-2).Entities:
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Year: 1988 PMID： 3361130 DOI： 10.1016/0022-1759(88)90239-6
Source DB: PubMed Journal: J Immunol Methods ISSN： 0022-1759 Impact factor: 2.303