Yingmin Wang1, Lijuan Gao2, Zhili Li3, Xingyou Ma4. 1. Department of Nursing, Xingtai Medical College, Hebei, 054000, China. 2. Department of Clinical, Xingtai Medical College, Hebei, 054000, China. 3. Department of Physiology, Hebei University of Chinese Medicine, Xingyuan Road No. 3, Hebei, 050200, China. lili3003@yeah.net. 4. Department of Clinical Medicine, Second Affiliated Hospital of Xingtai Medical College, Hebei, 054000, China.
Abstract
OBJECTIVE AND DESIGN: Diabetic retinopathy (DR) is one of the most serious microvascular complications of diabetes mellitus (DM). MicroRNAs (miRNAs) have been discovered to play a crucial role in DR, but the mechanisms underlying the effects of miR-301a-3p on DR are poorly understood. This paper was designed to explore the possible role of miR-301a-3p in DR. METHODS: The diabetic rat model was established by a single intraperitoneal injection of streptozotocin (STZ). The effects of miR-301a-3p on the biological functions of HRECs were determined through a series of experiments in vitro/vivo. RESULTS: The results revealed that interference with miR-301a-3p could decrease the expressions of inflammatory factors and apoptosis in the retinal tissue of DR. Furthermore, it can alleviate the oxidative stress in DR serum, reduce VEGF expression, increase endothelial cell marker expression, and inhibit (High Glucose) HG-induced apoptosis of HRECs. Six-transmembrane epithelial antigen of prostate 4 (STEAP4) was the target of miR-301a-3p. All the effects of miR-301a-3p in DR model were reversed by STEAP4 inhibitor. CONCLUSION: miR-301a-3p promotes diabetic retinopathy via regulation of STEAP4. The findings in this study may provide a vital reference for the drug research and development in DR treatment.
OBJECTIVE AND DESIGN:Diabetic retinopathy (DR) is one of the most serious microvascular complications of diabetes mellitus (DM). MicroRNAs (miRNAs) have been discovered to play a crucial role in DR, but the mechanisms underlying the effects of miR-301a-3p on DR are poorly understood. This paper was designed to explore the possible role of miR-301a-3p in DR. METHODS: The diabeticrat model was established by a single intraperitoneal injection of streptozotocin (STZ). The effects of miR-301a-3p on the biological functions of HRECs were determined through a series of experiments in vitro/vivo. RESULTS: The results revealed that interference with miR-301a-3p could decrease the expressions of inflammatory factors and apoptosis in the retinal tissue of DR. Furthermore, it can alleviate the oxidative stress in DR serum, reduce VEGF expression, increase endothelial cell marker expression, and inhibit (High Glucose) HG-induced apoptosis of HRECs. Six-transmembrane epithelial antigen of prostate 4 (STEAP4) was the target of miR-301a-3p. All the effects of miR-301a-3p in DR model were reversed by STEAP4 inhibitor. CONCLUSION:miR-301a-3p promotes diabetic retinopathy via regulation of STEAP4. The findings in this study may provide a vital reference for the drug research and development in DR treatment.
Authors: T Qaum; Q Xu; A M Joussen; M W Clemens; W Qin; K Miyamoto; H Hassessian; S J Wiegand; J Rudge; G D Yancopoulos; A P Adamis Journal: Invest Ophthalmol Vis Sci Date: 2001-09 Impact factor: 4.799
Authors: Polyxenie E Spoerri; Aqeela Afzal; Sergio Li Calzi; Lynn C Shaw; Jun Cai; Hao Pan; Michael Boulton; Maria B Grant Journal: Mol Vis Date: 2006-01-12 Impact factor: 2.367